Confocal laser endomicroscopy for in vivo diagnosis of Clostridium difficile associated colitis - a pilot study.

Günther C, Neumann H, Vieth M, Mudter J, Becker C, Schoerner C, Atreya R (2013)

Publication Language: English

Publication Status: Published

Publication Type: Journal article

Publication year: 2013

Journal

PLoS ONE Public Library of Science

Book Volume: 8

Pages Range: e58753

Journal Issue: 3

DOI: 10.1371/journal.pone.0058753

Abstract

Clostridium difficile infection (CDI) is one of the most dreaded causes of hospital-acquired diarrhea. Main objective was to investigate whether confocal laser endomicroscopy (CLE) has the capability for in vivo diagnosis of C. difficile associated histological changes. Second objective was to prove the presence of intramucosal bacteria using CLE.\n80 patients were prospectively included, 10 patients were diagnosed with CDI based on toxigenic culture. To validate the presence of intramucosal bacteria ex vivo, CLE was performed in pure C. difficile culture; additionally fluorescence in situ hybridization (FISH) was performed. Finally, CLE with fluorescence labelled oligonucleotide probe specific for C. difficile was performed ex vivo in order to prove the presence of bacteria.\nCLE identified CDI-associated histological changes in vivo (sensitivity and accuracy of 88.9% and 96.3%). In addition, intramucosal bacteria were visualized. The presence of these bacteria could be proven by CLE with labeled, specific molecular C. difficile probe and FISH-technique. Based on comparison between CLE and FISH analyses, sensitivity and specificity for the presence of intramucosal bacteria were 100%.\nCLE has the potential for in vivo diagnosis of CDI associated colitis. In addition, CLE allowed the detection of intramucosal bacteria in vivo.\nBACKGROUND\nMETHODS\nRESULTS\nCONCLUSION

Authors with CRIS profile

Claudia Günther Professur für Gastrointestinale Pathophysiologie Michael Vieth Medizinische Fakultät Christoph Becker Professur für Molekulare Gastroenterologie Raja Atreya Professur für Translationale Immunforschung bei chronisch entzündlichen Darmerkrankungen (Heisenberg-Professur)

Involved external institutions

HELIOS Kliniken DE Germany (DE)

How to cite

APA:

Günther, C., Neumann, H., Vieth, M., Mudter, J., Becker, C., Schoerner, C., & Atreya, R. (2013). Confocal laser endomicroscopy for in vivo diagnosis of Clostridium difficile associated colitis - a pilot study. PLoS ONE, 8(3), e58753. https://doi.org/10.1371/journal.pone.0058753

MLA:

Günther, Claudia, et al. "Confocal laser endomicroscopy for in vivo diagnosis of Clostridium difficile associated colitis - a pilot study." PLoS ONE 8.3 (2013): e58753.

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