Jetzschmann KJ, Yarman A, Rustam L, Kielb P, Urlacher VB, Fischer A, Weidinger IM, Wollenberger U, Scheller FW (2018)
Publication Type: Journal article
Publication year: 2018
Book Volume: 164
Pages Range: 240-246
DOI: 10.1016/j.colsurfb.2018.01.047
Hypothesis: Electrosynthesis of the MIP nano-film after binding of the separated domains or holo-cytochrome BM3 via an engineered anchor should result in domain-specific cavities in the polymer layer. Experiments: Both the two domains and the holo P450 BM3 have been bound prior polymer deposition via a N-terminal engineered his6-anchor to the electrode surface. Each step of MIP preparation was characterized by cyclic voltammetry of the redox-marker ferricyanide. Rebinding after template removal was evaluated by quantifying the suppression of the diffusive permeability of the signal for ferricyanide and by the NADH-dependent reduction of cytochrome c by the reductase domain (BMR). Findings: The working hypothesis is verified by the discrimination of the two domains by the respective MIPs: The holoenzyme P450 BM3 was ca. 5.5 times more effectively recognized by the film imprinted with the oxidase domain (BMO) as compared to the BMR-MIP or the non-imprinted polymer (NIP). Obviously, a cavity is formed during the imprinting process around the his
APA:
Jetzschmann, K.J., Yarman, A., Rustam, L., Kielb, P., Urlacher, V.B., Fischer, A.,... Scheller, F.W. (2018). Molecular LEGO by domain-imprinting of cytochrome P450 BM3. Colloids and Surfaces B: Biointerfaces, 164, 240-246. https://doi.org/10.1016/j.colsurfb.2018.01.047
MLA:
Jetzschmann, K. J., et al. "Molecular LEGO by domain-imprinting of cytochrome P450 BM3." Colloids and Surfaces B: Biointerfaces 164 (2018): 240-246.
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