Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics
Jiao C, Obst F, Geisler M, Che Y, Richter A, Appelhans D, Gaitzsch J, Voit B (2022)
Publication Type: Journal article
Publication year: 2022
Journal
Book Volume: 14
Article Number: 267
Journal Issue: 2
Abstract
Stimuli-responsive hydrogels have a wide range of potential applications in microflu-idics, which has drawn great attention. Double cross-linked hydrogels are very well suited for this application as they offer both stability and the required responsive behavior. Here, we re-port the integration of poly(N-isopropylacrylamide) (PNiPAAm) hydrogel with a permanent cross-linker (N,N′-methylenebisacrylamide, BIS) and a redox responsive reversible cross-linker (N,N′-bis(acryloyl)cystamine, BAC) into a microfluidic device through photopolymerization. Cleavage and re-formation of disulfide bonds introduced by BAC changed the cross-linking densities of the hydro-gel dots, making them swell or shrink. Rheological measurements allowed for selecting hydrogels that withstand long-term shear forces present in microfluidic devices under continuous flow. Once implemented, the thiol-disulfide exchange allowed the hydrogel dots to successfully capture and release the protein bovine serum albumin (BSA). BSA was labeled with rhodamine B and functionalized with 2-(2-pyridyldithio)-ethylamine (PDA) to introduce disulfide bonds. The reversible capture and release of the protein reached an efficiency of 83.6% in release rate and could be repeated over 3 cycles within the microfluidic device. These results demonstrate that our redox-responsive hydrogel dots enable the dynamic capture and release of various different functionalized (macro)molecules (e.g., proteins and drugs) and have a great potential to be integrated into a lab-on-a-chip device for detection and/or delivery.
Involved external institutions
Leibniz-Institut für Polymerforschung Dresden e. V.
Germany (DE)
Technische Universität Dresden
Germany (DE)
Germany (DE)
Technische Universität Dresden
Germany (DE)
How to cite
APA:
Jiao, C., Obst, F., Geisler, M., Che, Y., Richter, A., Appelhans, D.,... Voit, B. (2022). Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics. Polymers, 14(2). https://doi.org/10.3390/polym14020267
MLA:
Jiao, Chen, et al. "Reversible Protein Capture and Release by Redox-Responsive Hydrogel in Microfluidics." Polymers 14.2 (2022).
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