Chemiresistive biosensors based on carbon nanotubes for label-free detection of DNA sequences derived from avian influenza virus H5N1

Fu Y, Romay V, Liu Y, Ibarlucea B, Baraban L, Khavrus V, Oswald S, Bachmatiuk A, Ibrahim I, Ruemmeli M, Gemming T, Bezugly V, Cuniberti G (2017)

Publication Type: Journal article

Publication year: 2017

Journal

Sensors and Actuators B-Chemical Elsevier

Book Volume: 249

Pages Range: 691-699

DOI: 10.1016/j.snb.2017.04.080

Abstract

We developed chemiresistor-type biosensors based on carbon nanotubes for highly efficient and fast detection of avian influenza virus (AIV) subtype H5N1 DNA sequences. Semiconducting single-walled carbon nanotubes (sc-SWCNTs) or nitrogen-doped multi-walled carbon nanotubes (N-MWCNTs) were used as two alternative active sensing elements, and their sensitivity to different concentrations of DNA target were compared. In these sensors long nanotubes (>5 μm) were placed between interdigitated metal electrodes so that individual nanotubes connect the electrodes. The nanotubes were functionalized with DNA probe sequences non-covalently attached to the sidewalls. Such functionalized-nanotube sensors could reliably detect complementary DNA target sequences of the AIV H5N1 with concentration ranging from 2 pM to 2 nM in 15 min and at room temperature. Our nanotube-based biosensors are small, flexible, disposable and easy-to-fabricate that makes them promising for point-of-care applications and clinical diagnostics.

Involved external institutions

Technische Universität Dresden DE Germany (DE) Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW) / Leibniz Institute for Solid State and Materials Research Dresden DE Germany (DE)

How to cite

APA:

Fu, Y., Romay, V., Liu, Y., Ibarlucea, B., Baraban, L., Khavrus, V.,... Cuniberti, G. (2017). Chemiresistive biosensors based on carbon nanotubes for label-free detection of DNA sequences derived from avian influenza virus H5N1. Sensors and Actuators B-Chemical, 249, 691-699. https://doi.org/10.1016/j.snb.2017.04.080

MLA:

Fu, Yangxi, et al. "Chemiresistive biosensors based on carbon nanotubes for label-free detection of DNA sequences derived from avian influenza virus H5N1." Sensors and Actuators B-Chemical 249 (2017): 691-699.

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