Tailoring the Adsorption of ACE-Inhibiting Peptides by Nitrogen Functionalization of Porous Carbons
Huettner C, Hagemann D, Troschke E, Hippauf F, Borchardt L, Oswald S, Henle T, Kaskel S (2019)
Publication Type: Journal article
Publication year: 2019
Journal
Book Volume: 35
Pages Range: 9721-9731
Journal Issue: 30
DOI: 10.1021/acs.langmuir.9b00996
Abstract
Bioactive peptides, such as isoleucyl-tryptophan (IW), exhibit a high potential to inhibit the angiotensin-converting enzyme (ACE). Adsorption on carbon materials provides a beneficial method to extract these specific molecules from the complex mixture of an α-lactalbumin hydrolysate. This study focuses on the impact of nitrogen functionalization of porous carbon adsorbents, either via pre- or post-treatment, on the adsorption behavior of the ACE-inhibiting peptide IW and the essential amino acid tryptophan (W). The commercially activated carbon Norit ROX 0.8 is compared with pre- and postsynthetically functionalized N-doped carbon in terms of surface area, pore size, and surface functionality. For prefunctionalization, a covalent triazine framework was synthesized by trimerization of an aromatic nitrile under ionothermal conditions. For the postsynthetic approach, the activated carbon ROX 0.8 was functionalized with the nitrogen-rich molecule melamine. The batch adsorption results using model mixtures containing the single components IW and W could be transferred to a more complex mixture of an α-lactalbumin hydrolysate containing a huge number of various peptides. For this purpose, reverse-phase high-pressure liquid chromatography with fluorescence detection was used for identification and quantification. The treatment with the three different carbon materials leads to an increase in the ACE-inhibiting effect in vitro. The modified surface structure of the carbon via pre- or post-treatment allows separation of IW and W due to the certain selectivity for either the amino acid or the dipeptide.
Involved external institutions
Technische Universität Dresden
Germany (DE)
Fraunhofer-Institut für Werkstoff- und Strahltechnik (IWS) / Fraunhofer Institute for Material and Beam Technology
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW) / Leibniz Institute for Solid State and Materials Research Dresden
Germany (DE)
Germany (DE)
Fraunhofer-Institut für Werkstoff- und Strahltechnik (IWS) / Fraunhofer Institute for Material and Beam Technology
Germany (DE)
Ruhr-Universität Bochum (RUB)
Germany (DE)
Leibniz-Institut für Festkörper- und Werkstoffforschung Dresden e.V. (IFW) / Leibniz Institute for Solid State and Materials Research Dresden
Germany (DE)
How to cite
APA:
Huettner, C., Hagemann, D., Troschke, E., Hippauf, F., Borchardt, L., Oswald, S.,... Kaskel, S. (2019). Tailoring the Adsorption of ACE-Inhibiting Peptides by Nitrogen Functionalization of Porous Carbons. Langmuir, 35(30), 9721-9731. https://doi.org/10.1021/acs.langmuir.9b00996
MLA:
Huettner, Christiane, et al. "Tailoring the Adsorption of ACE-Inhibiting Peptides by Nitrogen Functionalization of Porous Carbons." Langmuir 35.30 (2019): 9721-9731.
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