Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation
Jain A, Ulman V, Mukherjee A, Prakash M, Cuenca MB, Pimpale LG, Münster S, Haase R, Panfilio KA, Jug F, Grill SW, Tomancak P, Pavlopoulos A (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 11
Article Number: 5604
Journal Issue: 1
DOI: 10.1038/s41467-020-19356-x
Abstract
Many animal embryos pull and close an epithelial sheet around the ellipsoidal egg surface during a gastrulation process known as epiboly. The ovoidal geometry dictates that the epithelial sheet first expands and subsequently compacts. Moreover, the spreading epithelium is mechanically stressed and this stress needs to be released. Here we show that during extraembryonic tissue (serosa) epiboly in the insect Tribolium castaneum, the non-proliferative serosa becomes regionalized into a solid-like dorsal region with larger non-rearranging cells, and a more fluid-like ventral region surrounding the leading edge with smaller cells undergoing intercalations. Our results suggest that a heterogeneous actomyosin cable contributes to the fluidization of the leading edge by driving sequential eviction and intercalation of individual cells away from the serosa margin. Since this developmental solution utilized during epiboly resembles the mechanism of wound healing, we propose actomyosin cable-driven local tissue fluidization as a conserved morphogenetic module for closure of epithelial gaps.
Authors with CRIS profile
Involved external institutions
Max-Planck-Institut für Physik komplexer Systeme (MPI PKS) / Max Planck Institute for the Physics of Complex Systems
Germany (DE)
Howard Hughes Medical Institute
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Universität zu Köln
Germany (DE)
Germany (DE)
Howard Hughes Medical Institute
United States (USA) (US)
Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Germany (DE)
Universität zu Köln
Germany (DE)
How to cite
APA:
Jain, A., Ulman, V., Mukherjee, A., Prakash, M., Cuenca, M.B., Pimpale, L.G.,... Pavlopoulos, A. (2020). Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation. Nature Communications, 11(1). https://doi.org/10.1038/s41467-020-19356-x
MLA:
Jain, Akanksha, et al. "Regionalized tissue fluidization is required for epithelial gap closure during insect gastrulation." Nature Communications 11.1 (2020).
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