The Cell Fate Controlling CLE40 Peptide Requires CNGCs to Trigger Highly Localized Ca2+ Transients in Arabidopsis thaliana Root Meristems

Breiden M, Olsson V, Bluemke P, Schlegel J, Gustavo-Pinto K, Dietrich P, Butenko MA, Simon R (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Plant and Cell Physiology Oxford University Press (OUP): Policy B - Oxford Open Option B

Book Volume: 62

Pages Range: 1290-1301

Journal Issue: 8

DOI: 10.1093/pcp/pcab079

Abstract

Communication between plant cells and their biotic environment largely depends on the function of plasma membrane localized receptor-like kinases (RLKs). Major players in this communication within root meristems are secreted peptides, including CLAVATA3/EMBRYO SURROUNDING REGION40 (CLE40). In the distal root meristem, CLE40 acts through the RLK ARABIDOPSIS CRINKLY4 (ACR4) and the leucine-rich repeat (LRR) RLK CLAVATA1 (CLV1) to promote cell differentiation. In the proximal meristem, CLE40 signaling requires the LRR receptor-like protein CLAVATA2 (CLV2) and the membrane localized pseudokinase CORYNE (CRN) and serves to inhibit cell differentiation. The molecular components that act immediately downstream of the CLE40-activated receptors are not yet known. Here, we show that active CLE40 signaling triggers the release of intracellular Ca2+ leading to increased cytosolic Ca2+ concentration ([Ca2+](cyt)) in a small subset of proximal root meristem cells. This rise in [Ca2+]cyt depends on the CYCLIC NUCLEOTIDE GATED CHANNELS (CNGCs) 6 and 9 and on CLV1. The precise function of changes in [Ca2+]cyt is not yet known but might form a central part of a fine-tuned response to CLE40 peptide that serves to integrate root meristem growth with stem cell fate decisions and initiation of lateral root primordia.

Authors with CRIS profile

Petra Dietrich Professur für Zellbiologie der Pflanzen

Involved external institutions

Heinrich-Heine-Universität Düsseldorf DE Germany (DE) University of Oslo NO Norway (NO)

How to cite

APA:

Breiden, M., Olsson, V., Bluemke, P., Schlegel, J., Gustavo-Pinto, K., Dietrich, P.,... Simon, R. (2021). The Cell Fate Controlling CLE40 Peptide Requires CNGCs to Trigger Highly Localized Ca2+ Transients in Arabidopsis thaliana Root Meristems. Plant and Cell Physiology, 62(8), 1290-1301. https://dx.doi.org/10.1093/pcp/pcab079

MLA:

Breiden, Maike, et al. "The Cell Fate Controlling CLE40 Peptide Requires CNGCs to Trigger Highly Localized Ca2+ Transients in Arabidopsis thaliana Root Meristems." Plant and Cell Physiology 62.8 (2021): 1290-1301.

BibTeX: Download