Pacquelet A, Zanin E, Ashiono C, Gotta M (2008)
Publication Status: Published
Publication Type: Journal article
Publication year: 2008
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Book Volume: 319
Pages Range: 267-272
Journal Issue: 2
DOI: 10.1016/j.ydbio.2008.04.016
The PAR proteins have an essential and conserved function in establishing polarity in many cell types and organisms. However, their key upstream regulators remain to be identified. In C. elegans, regulators of the PAR proteins can be identified by their ability to suppress the lethality of par-2 mutant embryos. Here we show that a nos-3 loss of function mutant suppresses the lethality of par-2 mutants by regulating PAR-6 protein levels. The suppression requires the activity of the sex determination genes fem-1/2/3 and of the cullin cul-2. FEM-1 is a substrate-specific adaptor for a CUL-2-based ubiquitin ligase (CBCFEM-1). Interestingly, we find that CUL-2 is required for the regulation of PAR-6 levels and that PAR-6 physically interacts with FEM-1. Our data strongly suggest that PAR-6 levels are regulated by the CBCFEM-1 ubiquitin ligase thereby uncovering a novel role for the FEM proteins and cullin-dependent degradation in regulating PAR proteins and polarity processes. (c) 2008 Elsevier Inc. All rights reserved.
APA:
Pacquelet, A., Zanin, E., Ashiono, C., & Gotta, M. (2008). PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditis elegans. Developmental Biology, 319(2), 267-272. https://dx.doi.org/10.1016/j.ydbio.2008.04.016
MLA:
Pacquelet, Anne, et al. "PAR-6 levels are regulated by NOS-3 in a CUL-2 dependent manner in Caenorhabditis elegans." Developmental Biology 319.2 (2008): 267-272.
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