Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons

Meltzer S, Yadav S, Lee J, Soba P, Younger SH, Jin P, Zhang W, Parrish J, Jan LY, Jan YN (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Neuron Elsevier (Cell Press)

Book Volume: 89

Pages Range: 741-755

Journal Issue: 4

DOI: 10.1016/j.neuron.2016.01.020

Abstract

Precise patterning of dendritic arbors is critical for the wiring and function of neural circuits. Dendrite-extracellular matrix (ECM) adhesion ensures that the dendrites of Drosophila dendritic arborization (da) sensory neurons are properly restricted in a 2D space, and thereby facilitates contact-mediated dendritic self-avoidance and tiling. However, the mechanisms regulating dendrite-ECM adhesion in vivo are poorly understood. Here, we show that mutations in the semaphorin ligand sema-2b lead to a dramatic increase in self-crossing of dendrites due to defects in dendrite-ECM adhesion, resulting in a failure to confine dendrites to a 2D plane. Furthermore, we find that Sema-2b is secreted from the epidermis and signals through the Plexin B receptor in neighboring neurons. Importantly, we find that Sema-2b/PlexB genetically and physically interacts with TORC2 complex, Tricornered (Trc) kinase, and integrins. These results reveal a novel role for semaphorins in dendrite patterning and illustrate how epidermal-derived cues regulate neural circuit assembly. Meltzer et al. demonstrate that semaphorin ligand derived from epidermis is required to restrict dendrites into a 2D space, thereby facilitating self-avoidance. They further show that Trc/Fry and integrin signaling pathways are components of Sema-2b/PlexB signaling.

Authors with CRIS profile

Peter Soba

Involved external institutions

University of California San Francisco (UCSF) US United States (USA) (US) University of Washington US United States (USA) (US) Universität Hamburg (UHH) DE Germany (DE)

How to cite

APA:

Meltzer, S., Yadav, S., Lee, J., Soba, P., Younger, S.H., Jin, P.,... Jan, Y.-N. (2016). Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons. Neuron, 89(4), 741-755. https://doi.org/10.1016/j.neuron.2016.01.020

MLA:

Meltzer, Shan, et al. "Epidermis-Derived Semaphorin Promotes Dendrite Self-Avoidance by Regulating Dendrite-Substrate Adhesion in Drosophila Sensory Neurons." Neuron 89.4 (2016): 741-755.

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