Usability of abattoir-acquired pig eyes for refractive excimer laser research

Topka M, Zhang Y, Bock A, Riedel P, Lörner J, Hammer A, Maier E, Paulsen F, Hammer C (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 11

Article Number: 19087

Journal Issue: 1

DOI: 10.1038/s41598-021-98635-z

Abstract

The purpose of this study was to elucidate, under which conditions abattoir-acquired pig eyes are suitable for refractive excimer laser experiments. Porcine eyes from tunnel-scalded (n = 5) and tank-scalded (n = 10) pigs were compared to unscalded eyes (n = 5) and to eyes scalded in the laboratory (n = 5). The corneal epithelium was removed before an excimer laser was used to perform a − 8.0 D photoablation. Corneal thickness was measured by optical coherence topography before and after photoablation. The ablation depth was determined with a contour measuring station, the morphology of the ablated areas was characterized by scanning electron microscopy and white-light profilometry. The scalded eyes showed an increase in corneal swelling which gained statistical significance in tank-scalded eyes showing a wedge-shaped opaque stromal lesion in the nasal corneal quadrant. A measurable deterioration of photoablation was only found in tank-scalded eyes that exhibited the opaque lesion. Ablated area morphology was smooth and regular in the unscalded and tunnel-scalded eyes. The tank-scalded eyes showed conspicuous wrinkles. While unscalded eyes should always be preferred for excimer laser laboratory experiments, the data suggest that the use of tunnel-scalded eyes may also be acceptable and should be chosen over tank-scalded eyes.

Authors with CRIS profile

Eva Maier Department of Operative Dentistry and Periodontology Friedrich Paulsen Lehrstuhl für Funktionelle und Klinische Anatomie Christian Hammer Lehrstuhl für Funktionelle und Klinische Anatomie

Involved external institutions

WaveLight GmbH DE Germany (DE) Paracelsus Medizinische Privatuniversität, Nürnberg DE Germany (DE)

How to cite

APA:

Topka, M., Zhang, Y., Bock, A., Riedel, P., Lörner, J., Hammer, A.,... Hammer, C. (2021). Usability of abattoir-acquired pig eyes for refractive excimer laser research. Scientific Reports, 11(1). https://doi.org/10.1038/s41598-021-98635-z

MLA:

Topka, Marius, et al. "Usability of abattoir-acquired pig eyes for refractive excimer laser research." Scientific Reports 11.1 (2021).

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