Heger L, Lühr JJ, Amon L, Smith AS, Eissing N, Dudziak D (2021)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2021
Publisher: Humana Press Inc.
Edited Volumes: Methods in Molecular Biology
Book Volume: 2350
Pages Range: 21-30
DOI: 10.1007/978-1-0716-1593-5_2
Confocal immunofluorescence microscopy is an advanced imaging technique routinely applied in the laboratory and clinics. Histological analyses are performed from tissue material. In general, a single fluorochrome per laser is employed, limiting simultaneous analysis to four antigens in one staining with a conventional 4-laser line microscope. Here, we describe a protocol for combining fluorochromes with the same excitation but different emission properties that allows for the analysis of six different antigens in confocal immunofluorescence microscopy with a conventional 4-laser line microscope. The proposed multiplexed method permits the identification and characterization of complex cell populations in rare tissue material.
APA:
Heger, L., Lühr, J.J., Amon, L., Smith, A.-S., Eissing, N., & Dudziak, D. (2021). Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines. In Methods in Molecular Biology. (pp. 21-30). Humana Press Inc..
MLA:
Heger, Lukas, et al. "Six-Color Confocal Immunofluorescence Microscopy with 4-Laser Lines." Methods in Molecular Biology. Humana Press Inc., 2021. 21-30.
BibTeX: Download