Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells
Schreibelt G, Benitez-Ribas D, Schuurhuis D, Lambeck AJA, Van Hout-Kuijer M, Schaft N, Punt CJA, Figdor CG, Adema GJ, De Vries IJM (2010)
Publication Type: Journal article
Publication year: 2010
Journal
Book Volume: 116
Pages Range: 564-574
Journal Issue: 4
DOI: 10.1182/blood-2009-11-251884
Abstract
Currently dendritic cell (DC)-based vaccines are explored in clinical trials, predominantly in cancer patients. Murine studies showed that only maturation with Toll-like receptor (TLR) ligands generates mature DCs that produce interleukin-12 and promote optimal T-cell help. Unfortunately, the limited availability of clinicalgrade TLR ligands significantly hampers the translation of these findings into DC-based vaccines. Therefore, we explored 15 commonly used preventive vaccines as a possible source of TLR ligands. We have identified a cocktail of the vaccines BCG-SSI, Influvac, and Typhim that contains TLR ligands and is capable of optimally maturing DCs. These DCs (vaccine DCs) showed high expression of CD80, CD86, and CD83 and secreted interleukin-12. Although vaccine DCs exhibited an impaired migratory capacity, this could be restored by addition of prostaglandin E2 (PGE 2; vaccine PGE2 DCs). Vaccine PGE2 DCs are potent inducers of T-cell proliferation and induce Th1 polarization. In addition, vaccine PGE2 DCs are potent inducers of tumor antigen-specific CD8 + effector T cells. Finally, vaccine PGE2-induced DC maturation is compatible with different antigen-loading strategies, including RNA electroporation. These data thus identify a new clinical application for a mixture of commonly used preventive vaccines in the generation of Th1-inducing clinical-grade mature DCs. © 2010 by The American Society of Hematology.
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APA:
Schreibelt, G., Benitez-Ribas, D., Schuurhuis, D., Lambeck, A.J.A., Van Hout-Kuijer, M., Schaft, N.,... De Vries, I.J.M. (2010). Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells. Blood, 116(4), 564-574. https://doi.org/10.1182/blood-2009-11-251884
MLA:
Schreibelt, Gerty, et al. "Commonly used prophylactic vaccines as an alternative for synthetically produced TLR ligands to mature monocyte-derived dendritic cells." Blood 116.4 (2010): 564-574.
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