Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy
Riet T, Holzinger A, Dörrie J, Schaft N, Schuler G, Abken H (2013)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2013
Journal
Publisher: Humana Press Inc.
Edited Volumes: Synthetic Messenger RNA and Cell Metabolism Modulation
Series: Methods in Molecular Biology
Book Volume: 969
Pages Range: 187-201
DOI: 10.1007/978-1-62703-260-5_12
Abstract
Redirecting T cells with a chimeric antigen receptor (CAR) of prede fined specificity showed remarkable ef ficacy in the adoptive therapy trials of malignant diseases. The CAR consists of a single chain fragment of variable region (scFv) antibody targeting domain covalently linked to the CD3 z signalling domain of the T cell receptor complex to mediate T cell activation upon antigen engagement. By using an antibodyderived targeting domain a CAR can potentially redirect T cells towards any target expressed on the cell surface as long as a binding domain is available. Antibody-mediated targeting moreover circumvents MHC restriction of the targeted antigen, thereby broadening the potential of applicability of adoptive T cell therapy. While T cells were so far genetically modi fied by viral transduction, transient modification with a CAR by RNA transfection gained increasing interest during the last years. This chapter focuses on methods to modify human T cells from peripheral blood with a CAR by electroporation of in vitro transcribed RNA and to test modi fied T cells for function for use in adoptive immunotherapy. © Springer Science+Business Media New York 2013.
Authors with CRIS profile
Jan Dörrie
Department of Dermatology
Niels Schaft
Medizinische Fakultät
Gerold Schuler
Lehrstuhl für Haut- und Geschlechtskrankheiten
Involved external institutions
Germany (DE)How to cite
APA:
Riet, T., Holzinger, A., Dörrie, J., Schaft, N., Schuler, G., & Abken, H. (2013). Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy. In Synthetic Messenger RNA and Cell Metabolism Modulation. (pp. 187-201). Humana Press Inc..
MLA:
Riet, Tobias, et al. "Nonviral RNA transfection to transiently modify T cells with chimeric antigen receptors for adoptive therapy." Synthetic Messenger RNA and Cell Metabolism Modulation. Humana Press Inc., 2013. 187-201.
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