Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells

Hoyer S, Gerer KF, Pfeiffer IA, Prommersberger S, Hoefflin S, Jaitly T, Beltrame L, Cavalieri D, Schuler G, Vera J, Schaft N, Dörrie J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Book Volume: 2015

Article Number: 952184

DOI: 10.1155/2015/952184

Abstract

For therapeutic cancer vaccination, the adoptive transfer of mRNA-electroporated dendritic cells (DCs) is frequently performed, usually with monocyte-derived, cytokine-matured DCs (moDCs). However, DCs are rich in danger-sensing receptors which could recognize the exogenously delivered mRNA and induce DC activation, hence influencing the DCs' immunogenicity. Therefore, we examined whether electroporation of mRNA with a proper cap and a poly-A tail of at least 64 adenosines had any influence on cocktail-matured moDCs. We used 16 different RNAs, encoding tumor antigens (MelanA, NRAS, BRAF, GNAQ, GNA11, and WT1), and variants thereof. None of those RNAs induced changes in the expression of CD25, CD40, CD83, CD86, and CD70 or the secretion of the cytokines IL-8, IL-6, and TNFα of more than 1.5-fold compared to the control condition, while an mRNA encoding an NF-B-activation protein as positive control induced massive secretion of the cytokines. To determine whether mRNA electroporation had any effect on the whole transcriptome of the DCs, we performed microarray analyses of DCs of 6 different donors. None of 60,000 probes was significantly different between mock-electroporated DCs and MelanA-transfected DCs. Hence, we conclude that no transcriptional programs were induced within cocktail-matured DCs by electroporation of single tumor-antigen-encoding mRNAs.

Authors with CRIS profile

Involved external institutions

Mario Negri Institute for Pharmacological Research (IRCCS) / Istituto di Ricerche Farmacologiche Mario Negri Italy (IT) Università degli Studi di Firenze / University of Florence Italy (IT)

How to cite

APA:

Hoyer, S., Gerer, K.F., Pfeiffer, I.A., Prommersberger, S., Hoefflin, S., Jaitly, T.,... Dörrie, J. (2015). Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells. Journal of Immunology Research, 2015. https://doi.org/10.1155/2015/952184

MLA:

Hoyer, Stefanie, et al. "Electroporated Antigen-Encoding mRNA Is Not a Danger Signal to Human Mature Monocyte-Derived Dendritic Cells." Journal of Immunology Research 2015 (2015).

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