Alternative splicing of pericentrin contributes to cell cycle control in cardiomyocytes

Steinfeldt J, Becker R, Vergarajauregui S, Engel F (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Cardiovascular Development and Disease MDPI

Book Volume: 8

Article Number: 87

Journal Issue: 8

DOI: 10.3390/jcdd8080087

Abstract

Induction of cardiomyocyte proliferation is a promising option to regenerate the heart. Thus, it is important to elucidate mechanisms that contribute to the cell cycle arrest of mammalian cardiomyocytes. Here, we assessed the contribution of the pericentrin (Pcnt) S isoform to cell cycle arrest in postnatal cardiomyocytes. Immunofluorescence staining of Pcnt isoforms combined with SiRNA-mediated depletion indicates that Pcnt S preferentially localizes to the nuclear envelope, while the Pcnt B isoform is enriched at centrosomes. This is further supported by the localization of ectopically expressed FLAG-tagged Pcnt S and Pcnt B in postnatal cardiomyocytes. Analysis of centriole configuration upon Pcnt depletion revealed that Pcnt B but not Pcnt S is required for centriole cohesion. Importantly, ectopic expression of Pcnt S induced centriole splitting in a heterologous system, ARPE-19 cells, and was sufficient to impair DNA synthesis in C2C12 myoblasts. Moreover, Pcnt S depletion enhanced serum-induced cell cycle re-entry in postnatal cardiomyocytes. Analysis of mitosis, binucleation rate, and cell number suggests that Pcnt S depletion enhances serum-induced progression of postnatal cardiomyocytes through the cell cycle resulting in cell division. Collectively, our data indicate that alternative splicing of Pcnt contributes to the establishment of cardiomyocyte cell cycle arrest shortly after birth.

Authors with CRIS profile

Jakob Steinfeldt Professur für Experimentelle Nieren- und Kreislaufforschung Robert Becker Department of Nephropathology Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

How to cite

APA:

Steinfeldt, J., Becker, R., Vergarajauregui, S., & Engel, F. (2021). Alternative splicing of pericentrin contributes to cell cycle control in cardiomyocytes. Journal of Cardiovascular Development and Disease, 8(8). https://doi.org/10.3390/jcdd8080087

MLA:

Steinfeldt, Jakob, et al. "Alternative splicing of pericentrin contributes to cell cycle control in cardiomyocytes." Journal of Cardiovascular Development and Disease 8.8 (2021).

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