Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina

Lux U, Ehrenberg J, Joachimsthaler A, Atorf J, Pircher B, Reim K, Kremers J, Gießl A, Brandstätter JH (2021)


Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 22

Article Number: 8131

Journal Issue: 15

DOI: 10.3390/ijms22158131

Abstract

Complexins (Cplxs) 1 to 4 are components of the presynaptic compartment of chemical synapses where they regulate important steps in synaptic vesicle exocytosis. In the retina, all four Cplxs are present, and while we know a lot about Cplxs 3 and 4, little is known about Cplxs 1 and 2. Here, we performed in situ hybridization experiments and bioinformatics and exploited Cplx 1 and Cplx 2 single-knockout mice combined with immunocytochemistry and light microscopy to characterize in detail the cell type and synapse-specific distribution of Cplx 1 and Cplx 2. We found that Cplx 2 and not Cplx 1 is the main isoform expressed in normal and displaced amacrine cells and ganglion cells in mouse retinae and that amacrine cells seem to operate with a single Cplx isoform at their conventional chemical synapses. Surprising was the finding that retinal function, determined with electroretinographic recordings, was altered in Cplx 1 but not Cplx 2 single-knockout mice. In summary, the results provide an important basis for future studies on the function of Cplxs 1 and 2 in the processing of visual signals in the mammalian retina.

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APA:

Lux, U., Ehrenberg, J., Joachimsthaler, A., Atorf, J., Pircher, B., Reim, K.,... Brandstätter, J.H. (2021). Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina. International Journal of Molecular Sciences, 22(15). https://doi.org/10.3390/ijms22158131

MLA:

Lux, Uwe, et al. "Cell types and synapses expressing the snare complex regulating proteins complexin 1 and complexin 2 in mammalian retina." International Journal of Molecular Sciences 22.15 (2021).

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