Anoctamin 6 is localized in the primary cilium of renal tubular cells and is involved in apoptosis-dependent cyst lumen formation

Forschbach V, Goppelt-Strübe M, Kunzelmann K, Schreiber R, Piedagnel R, Kraus A, Eckardt KU, Buchholz B (2015)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2015

Journal

Cell Death & Disease Nature Publishing Group: Open Access Journals - Option B / Nature Publishing Group

Publisher: NATURE PUBLISHING GROUP

Book Volume: 6

Article Number: ARTN e1899

DOI: 10.1038/cddis.2015.273

Abstract

Primary cilia are antenna-like structures projected from the apical surface of various mammalian cells including renal tubular cells. Functional or structural defects of the cilium lead to systemic disorders comprising polycystic kidneys as a key feature. Here we show that anoctamin 6 (ANO6), a member of the anoctamin chloride channel family, is localized in the primary cilium of renal epithelial cells in vitro and in vivo. ANO6 was not essential for cilia formation and had no effect on in vitro cyst expansion. However, knockdown of ANO6 impaired cyst lumen formation of MDCK cells in three-dimensional culture. In the absence of ANO6, apoptosis was reduced and epithelial cells were incompletely removed from the center of cell aggregates, which form in the early phase of cystogenesis. In line with these data, we show that ANO6 is highly expressed in apoptotic cyst epithelial cells of human polycystic kidneys. These data identify ANO6 as a cilium-associated protein and suggest its functional relevance in cyst formation.

Authors with CRIS profile

Margarete Goppelt-Strübe Department of Medicine 4 – Nephrology and Hypertension Andre Kraus Department of Medicine 4 – Nephrology and Hypertension Kai-Uwe Eckardt Department of Medicine 4 – Nephrology and Hypertension Björn Buchholz Department of Medicine 4 – Nephrology and Hypertension

Involved external institutions

University of Paris 6 - Pierre et Marie Curie / Université Paris VI Pierre et Marie Curie (UPMC) FR France (FR) Universität Regensburg DE Germany (DE)

How to cite

APA:

Forschbach, V., Goppelt-Strübe, M., Kunzelmann, K., Schreiber, R., Piedagnel, R., Kraus, A.,... Buchholz, B. (2015). Anoctamin 6 is localized in the primary cilium of renal tubular cells and is involved in apoptosis-dependent cyst lumen formation. Cell Death & Disease, 6. https://doi.org/10.1038/cddis.2015.273

MLA:

Forschbach, V., et al. "Anoctamin 6 is localized in the primary cilium of renal tubular cells and is involved in apoptosis-dependent cyst lumen formation." Cell Death & Disease 6 (2015).

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