An inwardly rectifying whole cell current induced by G(q)-coupled receptors
Buchholz B, Tauber R, Steffl D, Walz G, Koettgen M (2004)
Publication Status: Published
Publication Type: Journal article, Original article
Publication year: 2004
Journal
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Book Volume: 322
Pages Range: 177-185
Journal Issue: 1
DOI: 10.1016/j.bbrc.2004.07.103
Abstract
Ca2+ influx across the plasma membrane after stimulation of G protein-coupled receptors is important for many physiological functions. Here we studied the regulation of an inwardly rectifying whole cell current and its putative role in Ca2+ entry in Xenopus oocytes. Expression of P2Y(1) or M1 receptors in Xenopus oocytes elicited a characteristic inwardly rectifying current without receptor stimulation. This current displayed distinct activation and inactivation kinetics and was highly Ca2+-dependent. After stimulation of endogenous G(q)-coupled receptors in water-injected cells similar currents were observed. We therefore speculated that the current could be activated via Ca2+ store depletion induced by constitutive stimulation of the IP3 cascade in cells overexpressing G(q)-coupled receptors. Receptor-independent Ca2+ store depletion also induced the current. In conclusion, this current is activated after store depletion suggesting a role in Ca2+ entry after stimulation of G(q)-coupled receptors. Finally, our data do not support the proposed ionotropic properties of the P2Y(1) receptor. (C) 2004 Elsevier Inc. All rights reserved.
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Germany (DE)How to cite
APA:
Buchholz, B., Tauber, R., Steffl, D., Walz, G., & Koettgen, M. (2004). An inwardly rectifying whole cell current induced by G(q)-coupled receptors. Biochemical and Biophysical Research Communications, 322(1), 177-185. https://doi.org/10.1016/j.bbrc.2004.07.103
MLA:
Buchholz, Björn, et al. "An inwardly rectifying whole cell current induced by G(q)-coupled receptors." Biochemical and Biophysical Research Communications 322.1 (2004): 177-185.
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