Implementation of an enclosed ionization interface for the analysis of liquid sample streams with direct analysis in real time mass spectrometry

Kloth R, Khanipour P, Mayrhofer K, Katsounaros I (2021)


Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 35

Article Number: e9091

Journal Issue: 13

DOI: 10.1002/rcm.9091

Abstract

Rationale: The development of an interface to analyze liquid sample streams with direct analysis in real time mass spectrometry (DART-MS) is of great interest for coupling various analytical techniques, using non-volatile salts, with MS. Therefore, we devised an enclosed ionization interface and a sample introduction system for the versatile analysis of liquid samples with DART-MS. Methods: The sample introduction system consists of a nebulizer, a spray chamber and a transfer line, while the confined ionization interface is created by implementing a cross-shaped housing between ion source outlet and mass spectrometer inlet. Methodical studies of the effects of various setup parameters on signal intensity and peak shape were conducted, while its diverse applicability was demonstrated by coupling with high-performance liquid chromatography (HPLC) for the analysis of alcohols, organic acids and furanic compounds. Results: The confinement of the ionization interface results in a robust setup design with a well-defined ionization region for focusing of the sprayed sample mist. Thereby, an increase in analyte signal intensity by three orders of magnitude and improved signal stability and reproducibility were obtained in comparison with a similar open ionization interface configuration. Additionally, the successful quantification of alcohols could be demonstrated as well as the compatibility of the setup with HPLC gradient elution. Conclusions: A versatile setup design for the analysis of liquid sample streams with DART-MS was devised for monitoring reactions or hyphenating analytics with MS. The design minimizes interferences from the laboratory surroundings as well as allows for safe handling of hazardous and toxic chemicals, which renders it suitable for a broad range of applications.

Authors with CRIS profile

Involved external institutions

How to cite

APA:

Kloth, R., Khanipour, P., Mayrhofer, K., & Katsounaros, I. (2021). Implementation of an enclosed ionization interface for the analysis of liquid sample streams with direct analysis in real time mass spectrometry. Rapid Communications in Mass Spectrometry, 35(13). https://doi.org/10.1002/rcm.9091

MLA:

Kloth, Ricarda, et al. "Implementation of an enclosed ionization interface for the analysis of liquid sample streams with direct analysis in real time mass spectrometry." Rapid Communications in Mass Spectrometry 35.13 (2021).

BibTeX: Download