Binding Mechanism of the Model Charged Dye Carboxyfluorescein to Hyaluronan/Polylysine Multilayers

Prokopovic VZ, Vikulina AS, Sustr D, Shchukina EM, Shchukin DG, Volodkin DV (2017)

Publication Type: Journal article

Publication year: 2017

Journal

ACS Applied Materials and Interfaces American Chemical Society

Book Volume: 9

Pages Range: 38908-38918

Journal Issue: 44

DOI: 10.1021/acsami.7b12449

Abstract

Biopolymer-based multilayers become more and more attractive due to the vast span of biological application they can be used for, e.g., implant coatings, cell culture supports, scaffolds. Multilayers have demonstrated superior capability to store enormous amounts of small charged molecules, such as drugs, and release them in a controlled manner; however, the binding mechanism for drug loading into the multilayers is still poorly understood. Here we focus on this mechanism using model hyaluronan/polylysine (HA/PLL) multilayers and a model charged dye, carboxyfluorescein (CF). We found that CF reaches a concentration of 13 mM in the multilayers that by far exceeds its solubility in water. The high loading is not related to the aggregation of CF in the multilayers. In the multilayers, CF molecules bind to free amino groups of PLL; however, intermolecular CF-CF interactions also play a role and (i) endow the binding with a cooperative nature and (ii) result in polyadsorption of CF molecules, as proven by fitting of the adsorption isotherm using the BET model. Analysis of CF mobility in the multilayers by fluorescence recovery after photobleaching has revealed that CF diffusion in the multilayers is likely a result of both jumping of CF molecules from one amino group to another and movement, together with a PLL chain being bound to it. We believe that this study may help in the design of tailor-made multilayers that act as advanced drug delivery platforms for a variety of bioapplications where high loading and controlled release are strongly desired.

Authors with CRIS profile

Anna Vikulina

Involved external institutions

Fraunhofer-Institut für Zelltherapie und Immunologie (IZI) DE Germany (DE) The University of Liverpool GB United Kingdom (GB) Nottingham Trent University GB United Kingdom (GB)

How to cite

APA:

Prokopovic, V.Z., Vikulina, A.S., Sustr, D., Shchukina, E.M., Shchukin, D.G., & Volodkin, D.V. (2017). Binding Mechanism of the Model Charged Dye Carboxyfluorescein to Hyaluronan/Polylysine Multilayers. ACS Applied Materials and Interfaces, 9(44), 38908-38918. https://doi.org/10.1021/acsami.7b12449

MLA:

Prokopovic, Vladimir Z., et al. "Binding Mechanism of the Model Charged Dye Carboxyfluorescein to Hyaluronan/Polylysine Multilayers." ACS Applied Materials and Interfaces 9.44 (2017): 38908-38918.

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