Bioactivity of catalase loaded into vaterite CaCO3 crystals via adsorption and co-synthesis
Feoktistova NA, Vikulina AS, Balabushevich NG, Skirtach AG, Volodkin D (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 185
Article Number: 108223
DOI: 10.1016/j.matdes.2019.108223
Abstract
Protein therapy gained a reputation of the most direct and safe approach for treating various diseases, yet biodegradation and loss of bioactivity of fragile therapeutic proteins limit their wide medical use. Recently, a new hard templating technology using decomposable mesoporous vaterite CaCO3 crystals became extremely popular strategy for formulation of protein nano(micro)-vectors. This study deciphers how protein bioactivity depends on protein loading/release for this technology utilizing catalase as a promising antioxidant therapeutic agent. Catalase has been loaded into CaCO3 using two approaches: i) passive - via adsorption (ADS) into pre-formed crystals and ii) active - via co-synthesis (COS) in the pH range 8–10. Crystal morphology, protein secondary structure and enzymatic bioactivity, and protein retention upon washing are assessed. The activity reduction (∼70% for COS and ∼20% for ADS) is caused by both protein exposure to an alkaline medium and protein aggregation induced by Ca2+. The aggregation significantly governs protein release kinetics. Catalase loading into the crystals is pH-independent and van der Waals interactions dominate over the electrostatics, while catalase activity strongly depends on pH. This study implicates the prime role of loading/release mechanism in the preservation of protein bioactivity and guide for the control over the retention of protein bioactivity.
Authors with CRIS profile
Involved external institutions
Universiteit Gent (UGent) / Ghent University
Belgium (BE)
Lomonosov Moscow State University / Московский государственный университет имени М.В.Ломоносова
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Belgium (BE)
Lomonosov Moscow State University / Московский государственный университет имени М.В.Ломоносова
Russian Federation (RU)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
How to cite
APA:
Feoktistova, N.A., Vikulina, A.S., Balabushevich, N.G., Skirtach, A.G., & Volodkin, D. (2020). Bioactivity of catalase loaded into vaterite CaCO3 crystals via adsorption and co-synthesis. Materials & Design, 185. https://doi.org/10.1016/j.matdes.2019.108223
MLA:
Feoktistova, Natalia A., et al. "Bioactivity of catalase loaded into vaterite CaCO3 crystals via adsorption and co-synthesis." Materials & Design 185 (2020).
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