TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis
Mangal S, Sacher J, Kim T, Osorio DS, Motegi F, Carvalho AX, Oegema K, Zanin E (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 217
Pages Range: 837-848
Journal Issue: 3
Abstract
During cytokinesis, a signal from the central spindle that forms between the separating anaphase chromosomes promotes the accumulation of contractile ring components at the cell equator, while a signal from the centrosomal microtubule asters inhibits accumulation of contractile ring components at the cell poles. However, the molecular identity of the inhibitory signal has remained unknown. To identify molecular components of the aster-based inhibitory signal, we developed a means to monitor the removal of contractile ring proteins from the polar cortex after anaphase onset. Using this assay, we show that polar clearing is an active process that requires activation of Aurora A kinase by TPXL-1. TPXL-1 concentrates on astral microtubules coincident with polar clearing in anaphase, and its ability to recruit Aurora A and activate its kinase activity are essential for clearing. In summary, our data identify Aurora A kinase as an aster-based inhibitory signal that restricts contractile ring components to the cell equator during cytokinesis.
Authors with CRIS profile
Additional Organisation(s)
Involved external institutions
Ludwig-Maximilians-Universität (LMU)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Universidade do Porto
Portugal (PT)
National University of Singapore (NUS)
Singapore (SG)
Germany (DE)
University of California, San Diego
United States (USA) (US)
Universidade do Porto
Portugal (PT)
National University of Singapore (NUS)
Singapore (SG)
How to cite
APA:
Mangal, S., Sacher, J., Kim, T., Osorio, D.S., Motegi, F., Carvalho, A.X.,... Zanin, E. (2018). TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis. The Journal of Cell Biology, 217(3), 837-848. https://dx.doi.org/10.1083/jcb.201706021
MLA:
Mangal, Sriyash, et al. "TPXL-1 activates Aurora A to clear contractile ring components from the polar cortex during cytokinesis." The Journal of Cell Biology 217.3 (2018): 837-848.
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