Allergenicity assessment of the edible cricket Acheta domesticus in terms of thermal and gastrointestinal processing and IgE cross-reactivity with shrimp
De Marchi L, Mainente F, Leonardi M, Scheurer S, Wangorsch A, Mahler V, Pilolli R, Sorio D, Zoccatelli G (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 359
Article Number: 129878
DOI: 10.1016/j.foodchem.2021.129878
Abstract
The allergenic potency of the cricket Acheta domesticus, a promising edible insect, has never been assessed. This work aims to study the immunoreactivity of Acheta domesticus, and its cross-reactivity with the shrimp Litopenaeus vannamei, assessing the effect of cooking and gastrointestinal digestion on their allergenic properties. Different cricket proteins were detected by immunoblotting with shrimp-allergic patients' sera. Tropomyosin was identified as the most relevant IgE-binding protein, and its cross-reactivity with shrimp tropomyosin was demonstrated by ELISA. While shrimp tropomyosin showed scarce stability to gastric digestion, cricket tropomyosin withstood the whole digestion process. The sarcoplasmic calcium-binding protein, specifically detected in shrimp, showed exceptional stability to gastrointestinal digestion. IgE-binding proteins in a model of enriched baked products were partially protected from proteolysis. In conclusion, the ingestion of A. domesticus proteins poses serious concerns to the Crustacean-allergic population. The high stability of tropomyosin may represent a risk of primary sensitization and clinical cross-reactivity.
Authors with CRIS profile
Involved external institutions
University of Verona / Università degli Studi di Verona
Italy (IT)
Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI)
Germany (DE)
Institute of Sciences of Food Production / Istituto di Scienze delle Produzioni Alimentari (ISPA-CNR)
Italy (IT)
Italy (IT)
Paul Ehrlich Institute / Paul-Ehrlich-Institut – Bundesinstitut für Impfstoffe und biomedizinische Arzneimittel (PEI)
Germany (DE)
Institute of Sciences of Food Production / Istituto di Scienze delle Produzioni Alimentari (ISPA-CNR)
Italy (IT)
How to cite
APA:
De Marchi, L., Mainente, F., Leonardi, M., Scheurer, S., Wangorsch, A., Mahler, V.,... Zoccatelli, G. (2021). Allergenicity assessment of the edible cricket Acheta domesticus in terms of thermal and gastrointestinal processing and IgE cross-reactivity with shrimp. Food Chemistry, 359. https://doi.org/10.1016/j.foodchem.2021.129878
MLA:
De Marchi, Laura, et al. "Allergenicity assessment of the edible cricket Acheta domesticus in terms of thermal and gastrointestinal processing and IgE cross-reactivity with shrimp." Food Chemistry 359 (2021).
BibTeX: Download