Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model
Horder H, Lasheras MG, Grummel N, Nadernezhad A, Herbig J, Erguen S, Tessmar J, Groll J, Fabry B, Bauer-Kreisel P, Blunk T (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 10
Journal Issue: 4
Abstract
Biofabrication, including printing technologies, has emerged as a powerful approach to the design of disease models, such as in cancer research. In breast cancer, adipose tissue has been acknowledged as an important part of the tumor microenvironment favoring tumor progression. Therefore, in this study, a 3D-printed breast cancer model for facilitating investigations into cancer cell-adipocyte interaction was developed. First, we focused on the printability of human adipose-derived stromal cell (ASC) spheroids in an extrusion-based bioprinting setup and the adipogenic differentiation within printed spheroids into adipose microtissues. The printing process was optimized in terms of spheroid viability and homogeneous spheroid distribution in a hyaluronic acid-based bioink. Adipogenic differentiation after printing was demonstrated by lipid accumulation, expression of adipogenic marker genes, and an adipogenic ECM profile. Subsequently, a breast cancer cell (MDA-MB-231) compartment was printed onto the adipose tissue constructs. After nine days of co-culture, we observed a cancer cell-induced reduction of the lipid content and a remodeling of the ECM within the adipose tissues, with increased fibronectin, collagen I and collagen VI expression. Together, our data demonstrate that 3D-printed breast cancer-adipose tissue models can recapitulate important aspects of the complex cell-cell and cell-matrix interplay within the tumor-stroma microenvironment.
Authors with CRIS profile
Nadine Grummel
Sonderforschungsbereich/Transregio 225/2: Von den Grundlagen der Biofabrikation zu funktionalen Gewebemodellen
Ben Fabry
Lehrstuhl für Biophysik
Involved external institutions
Germany (DE)How to cite
APA:
Horder, H., Lasheras, M.G., Grummel, N., Nadernezhad, A., Herbig, J., Erguen, S.,... Blunk, T. (2021). Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model. Cells, 10(4). https://doi.org/10.3390/cells10040803
MLA:
Horder, Hannes, et al. "Bioprinting and Differentiation of Adipose-Derived Stromal Cell Spheroids for a 3D Breast Cancer-Adipose Tissue Model." Cells 10.4 (2021).
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