Imaging-Based Screening Platform Assists Protein Engineering

Fabritius A, Ng D, Kist AM, Erdogan M, Portugues R, Griesbeck O (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Cell Chemical Biology Elsevier Science

Book Volume: 25

Pages Range: 1554-1561.e8

Journal Issue: 12

DOI: 10.1016/j.chembiol.2018.08.008

Abstract

Protein evolution requires generation and screening of large numbers of variants. To expedite screening, Fabritius et al. built a platform that integrates fluorescence-based image analysis and picking of bacterial colonies. They engineered mCarmine, a far-red-emitting fluorescent protein for tagging and deep tissue imaging.

Authors with CRIS profile

Andreas Kist

Involved external institutions

Max-Planck-Institut für Neurobiologie / Max Planck Institute of Neurobiology DE Germany (DE)

How to cite

APA:

Fabritius, A., Ng, D., Kist, A.M., Erdogan, M., Portugues, R., & Griesbeck, O. (2018). Imaging-Based Screening Platform Assists Protein Engineering. Cell Chemical Biology, 25(12), 1554-1561.e8. https://dx.doi.org/10.1016/j.chembiol.2018.08.008

MLA:

Fabritius, Arne, et al. "Imaging-Based Screening Platform Assists Protein Engineering." Cell Chemical Biology 25.12 (2018): 1554-1561.e8.

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