Weitkamp JT, Wöltje M, Nußpickel B, Schmidt FN, Aibibu D, Bayer A, Eglin D, Armiento AR, Arnold P, Cherif C, Lucius R, Smeets R, Kurz B, Behrendt P (2021)
Publication Type: Journal article
Publication year: 2021
Book Volume: 22
Article Number: 3635
Journal Issue: 7
DOI: 10.3390/ijms22073635
A continuing challenge in cartilage tissue engineering for cartilage regeneration is the creation of a suitable synthetic microenvironment for chondrocytes and tissue regeneration. The aim of this study was to develop a highly tunable hybrid scaffold based on a silk fibroin matrix (SM) and a hyaluronic acid (HA) hydrogel. Human articular chondrocytes were embedded in a porous 3-dimensional SM, before infiltration with tyramine modified HA hydrogel. Scaffolds were cultured in chondropermissive medium with and without TGF-β1. Cell viability and cell distribution were assessed using CellTiter-Blue assay and Live/Dead staining. Chondrogenic marker expression was detected using qPCR. Biosynthesis of matrix compounds was analyzed by dimethylmethylene blue assay and immuno-histology. Differences in biomaterial stiffness and stress relaxation were characterized using a one-step unconfined compression test. Cell morphology was investigated by scanning electron microscopy. Hybrid scaffold revealed superior chondro-inductive and biomechanical properties compared to sole SM. The presence of HA and TGF-β1 increased chondrogenic marker gene expression and matrix deposition. Hybrid scaffolds offer cytocompatible and highly tunable properties as cell-carrier systems, as well as favorable biomechanical properties.
APA:
Weitkamp, J.T., Wöltje, M., Nußpickel, B., Schmidt, F.N., Aibibu, D., Bayer, A.,... Behrendt, P. (2021). Silk fiber-reinforced hyaluronic acid-based hydrogel for cartilage tissue engineering. International Journal of Molecular Sciences, 22(7). https://doi.org/10.3390/ijms22073635
MLA:
Weitkamp, Jan Tobias, et al. "Silk fiber-reinforced hyaluronic acid-based hydrogel for cartilage tissue engineering." International Journal of Molecular Sciences 22.7 (2021).
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