Enzyme-capped relay-functionalized mesoporous carbon nanoparticles: Effective bioelectrocatalytic matrices for sensing and biofuel cell applications

Trifonov A, Herkendell K, Tel-Vered R, Yehezkeli O, Woerner M, Willner I (2013)

Publication Type: Journal article

Publication year: 2013

Journal

ACS nano American Chemical Society

Book Volume: 7

Pages Range: 11358-11368

Journal Issue: 12

DOI: 10.1021/nn405218x

Abstract

The porous high surface area and conducting properties of mesoporous carbon nanoparticles, CNPs (<500 nm diameter of NPs, pore dimensions ∼6.3 nm), are implemented to design electrically contacted enzyme electrodes for biosensing and biofuel cell applications. The relay units ferrocene methanol, Fc-MeOH, methylene blue, MB⁺, and 2,2′-azinobis(3- ethylbenzothiazoline-6-sulfonic acid), ABTS^2-, are loaded in the pores of the mesoporous CNPs, and the pores are capped with glucose oxidase, GOx, horseradish peroxidase, HRP, or bilirubin oxidase, BOD, respectively. The resulting relay/enzyme-functionalized CNPs are immobilized on glassy carbon electrodes, and the relays encapsulated in the pores are sufficiently free to electrically contact the different enzymes with the bulk electrode supports. The Fc-MeOH/GOx CNP-functionalized electrode is implemented for the bioelectrocatalyzed sensing of glucose, and the MB⁺/HRP-modified CNPs are applied for the electrochemical sensing of H2O2. The ABTS^2-/BOD-modified CNPs provide an effective electrically contacted material for the bioelectrocatalyzed reduction of O2 (kcat = 94 electrons·s^-1). Integration of the Fc-MeOH/GOx CNP electrode and of the electrically wired ABTS^2-/BOD CNP electrode as anode and cathode, respectively, yields a biofuel cell revealing a power output of ∼95 μW·cm^-2. © 2013 American Chemical Society.

Authors with CRIS profile

Katharina Herkendell

Involved external institutions

Hebrew University of Jerusalem

Israel (IL) Karlsruhe Institute of Technology (KIT)

Germany (DE)

How to cite

APA:

Trifonov, A., Herkendell, K., Tel-Vered, R., Yehezkeli, O., Woerner, M., & Willner, I. (2013). Enzyme-capped relay-functionalized mesoporous carbon nanoparticles: Effective bioelectrocatalytic matrices for sensing and biofuel cell applications. ACS nano, 7(12), 11358-11368. https://dx.doi.org/10.1021/nn405218x

MLA:

Trifonov, Alexander, et al. "Enzyme-capped relay-functionalized mesoporous carbon nanoparticles: Effective bioelectrocatalytic matrices for sensing and biofuel cell applications." ACS nano 7.12 (2013): 11358-11368.

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