Pracht K, Meinzinger J, Schulz S, Daum P, Corte-Real J, Hauke M, Roth E, Kindermann D, Mielenz D, Schuh W, Wittmann J, Jäck HM (2021)
Publication Type: Journal article
Publication year: 2021
Long-lived antibody-secreting plasma cells are essential to establish humoral memory against pathogens. While a regulatory transcription factor network has been established in plasma cell differentiation, the regulatory role of miRNAs remains enigmatic. We have recently identified miR-148a as the most abundant miRNA in primary mouse and human plasma cells. To determine whether this plasma cell signature miRNA controls the in vivo development of B cells into long-lived plasma cells, we established mice with genomic, conditional, and inducible deletions of miR-148a. The analysis of miR-148a-deficient mice revealed reduced serum Ig, decreased numbers of newly formed plasmablasts and reduced CD19-negative, CD93-positive long-lived plasma cells. Transcriptome and metabolic analysis revealed an impaired glucose uptake, a reduced oxidative phosphorylation-based energy metabolism, and an altered abundance of homing receptors CXCR3 (increase) and CXCR4 (reduction) in miR-148a-deficient plasma cells. These findings support the role of miR-148a as a positive regulator of the maintenance of long-lived plasma cells.
APA:
Pracht, K., Meinzinger, J., Schulz, S., Daum, P., Corte-Real, J., Hauke, M.,... Jäck, H.-M. (2021). miR-148a controls metabolic programming and survival of mature CD19-negative plasma cells in mice. European Journal of Immunology. https://doi.org/10.1002/eji.202048993
MLA:
Pracht, Katharina, et al. "miR-148a controls metabolic programming and survival of mature CD19-negative plasma cells in mice." European Journal of Immunology (2021).
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