Identification and purification of novel low-molecular-weight lupine allergens as components for personalized diagnostics
Jappe U, Karstedt A, Warneke D, Hellmig S, Böttger M, Riffelmann FW, Treudler R, Lange L, Abraham S, Dölle-Bierke S, Worm M, Wagner N, Ruëff F, Reese G, Knulst AC, Becker WM (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 13
Pages Range: 1-21
Article Number: 409
Journal Issue: 2
DOI: 10.3390/nu13020409
Abstract
Lupine flour is a valuable food due to its favorable nutritional properties. In spite of its allergenic potential, its use is increasing. Three lupine species, Lupinus angustifolius, L. luteus, and L. albus are relevant for human nutrition. The aim of this study is to clarify whether the species differ with regard to their allergen composition and whether anaphylaxis marker allergens could be identified in lupine. Patients with the following characteristics were included: lupine allergy, suspected lupine allergy, lupine sensitization only, and peanut allergy. Lupine sensitization was detected via CAP-FEIA (ImmunoCAP) and skin prick test. Protein, DNA and expressed sequence tag (EST) databases were queried for lupine proteins homologous to already known legume allergens. Different extraction methods applied on seeds from all species were examined by SDS-PAGE and screened by immunoblotting for IgE-binding proteins. The extracts underwent different and successive chromatography methods. Low-molecular-weight components were purified and investigated for IgE-reactivity. Proteomics revealed a molecular diversity of the three species, which was confirmed when investigated for IgE-reactivity. Three new allergens, L. albus profilin, L. angustifolius and L. luteus lipid transfer protein (LTP), were identified. LTP as a potential marker allergen for severity is a valuable additional candidate for molecular allergy diagnostic tests.
Involved external institutions
Charité - Universitätsmedizin Berlin
Germany (DE)
Fachkrankenhaus Kloster Grafschaft
Germany (DE)
St.-Marien-Hospital Bonn
Germany (DE)
Universitätsklinikum Carl Gustav Carus Dresden
Germany (DE)
Deutsches Zentrum für Infektionsforschung (DZIF)
Germany (DE)
Klinikum der Universität München
Germany (DE)
ALLERGOPHARMA GmbH & Co. KG
Germany (DE)
Universität Leipzig
Germany (DE)
University Medical Centre Utrecht (UMC Utrecht)
Netherlands (NL)
Germany (DE)
Fachkrankenhaus Kloster Grafschaft
Germany (DE)
St.-Marien-Hospital Bonn
Germany (DE)
Universitätsklinikum Carl Gustav Carus Dresden
Germany (DE)
Deutsches Zentrum für Infektionsforschung (DZIF)
Germany (DE)
Klinikum der Universität München
Germany (DE)
ALLERGOPHARMA GmbH & Co. KG
Germany (DE)
Universität Leipzig
Germany (DE)
University Medical Centre Utrecht (UMC Utrecht)
Netherlands (NL)
How to cite
APA:
Jappe, U., Karstedt, A., Warneke, D., Hellmig, S., Böttger, M., Riffelmann, F.W.,... Becker, W.M. (2021). Identification and purification of novel low-molecular-weight lupine allergens as components for personalized diagnostics. Nutrients, 13(2), 1-21. https://doi.org/10.3390/nu13020409
MLA:
Jappe, Uta, et al. "Identification and purification of novel low-molecular-weight lupine allergens as components for personalized diagnostics." Nutrients 13.2 (2021): 1-21.
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