An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle

Schneidereit D, Broellochs A, Ritter P, Kreiß L, Mokhtari Z, Beilhack A, Krönke G, Ackermann J, Faas M, Grüneboom A, Schürmann S, Friedrich O (2021)

Publication Type: Journal article, Original article

Publication year: 2021

Journal

Theranostics Ivyspring International Publisher

Book Volume: 11

Pages Range: 2876-2891

Journal Issue: 6

DOI: 10.7150/thno.51558

Abstract

Rationale: Structural remodeling or damage as a result of disease or injury is often not evenly distributed throughout a tissue but strongly depends on localization and extent of damaging stimuli. Skeletal muscle as a mechanically active organ can express signs of local or even systemic myopathic damage, necrosis, or repair. Conventionally, muscle biopsies (patients) or whole muscles (animal models) are mechanically sliced and stained to assess structural alterations histologically. Three-dimensional tissue information can be obtained by applying deep imaging modalities, e.g. multiphoton or light-sheet microscopy. Chemical clearing approaches reduce scattering, e.g. through matching refractive tissue indices, to overcome optical penetration depth limits in thick tissues.

Authors with CRIS profile

Dominik Schneidereit Lehrstuhl für Medizinische Biotechnologie Paul Ritter Lehrstuhl für Medizinische Biotechnologie Lucas Kreiß Lehrstuhl für Medizinische Biotechnologie Gerhard Krönke Professur für Translationale Immunologie Jochen Ackermann Department of Medicine 3 – Rheumatology and Immunology Maria Faas Professur für Immunologie Sebastian Schürmann Lehrstuhl für Medizinische Biotechnologie Oliver Friedrich Lehrstuhl für Medizinische Biotechnologie

Involved external institutions

Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Schneidereit, D., Broellochs, A., Ritter, P., Kreiß, L., Mokhtari, Z., Beilhack, A.,... Friedrich, O. (2021). An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle. Theranostics, 11(6), 2876-2891. https://doi.org/10.7150/thno.51558

MLA:

Schneidereit, Dominik, et al. "An advanced optical clearing protocol allows label-free detection of tissue necrosis via multiphoton microscopy in injured whole muscle." Theranostics 11.6 (2021): 2876-2891.

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