The posttraumatic activation of CD4+ T regulatory cells is modulated by TNFR2- and TLR4-dependent pathways, but not by IL-10

Bock M, Bergmann CB, Jung S, Kalbitz M, Relja B, Huber-Wagnerd S, Biberthaler P, Van Griensven M, Hanschen M (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Cellular Immunology Elsevier

Book Volume: 331

Pages Range: 137-145

DOI: 10.1016/j.cellimm.2018.06.009

Abstract

Platelets modulate the immune system following injury by interacting with CD4+ T regulatory cells (CD4+ Tregs). The underlying mechanisms remain unsolved. We hypothesize paracrine interactions via Tumor necrosis factor-alpha (TNFα)-, Toll like receptor-4 (TLR4)-, and Interleukin-10 (IL-10). In the murine burn injury model, CD4+ Treg activation pathways were selectively addressed using TNFR2-, TLR4- and IL-10-deficient mice. The CD4+ Treg signalling molecule PKC-θ was analyzed using phospho-flow cytometry to detect rapid cell activation. Thromboelastometry (ROTEM®) was used to assess platelet activation. Injury induced significant early activation of CD4+ Tregs, disruption of TNFR2 and TLR4 activation pathways resulted in lower activity. The disruption of IL-10 crosstalk had no significant impact. Selective disruption of paracrine interactions is associated with changes in posttraumatic hemostasis parameters. TNFR2- and TLR4-dependent pathways modulate the activation of CD4+ Tregs following trauma. In contrast, we did not observe a role of IL-10 in the posttraumatic activation of CD4+ Tregs. One sentence summary: TLR4- and TNFR2-dependent mechanisms, but not IL-10-dependent pathways, modulate the anti-inflammatory response of CD4+ Tregs following trauma.

Authors with CRIS profile

Miriam Kalbitz

Involved external institutions

Goethe-Universität Frankfurt am Main DE Germany (DE) Technische Universität München (TUM) DE Germany (DE) Universität Ulm DE Germany (DE)

How to cite

APA:

Bock, M., Bergmann, C.B., Jung, S., Kalbitz, M., Relja, B., Huber-Wagnerd, S.,... Hanschen, M. (2018). The posttraumatic activation of CD4+ T regulatory cells is modulated by TNFR2- and TLR4-dependent pathways, but not by IL-10. Cellular Immunology, 331, 137-145. https://doi.org/10.1016/j.cellimm.2018.06.009

MLA:

Bock, Matthias, et al. "The posttraumatic activation of CD4+ T regulatory cells is modulated by TNFR2- and TLR4-dependent pathways, but not by IL-10." Cellular Immunology 331 (2018): 137-145.

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