Cardiac glucose and fatty acid transport after experimental mono- And polytrauma

Lackner I, Weber B, Knecht D, Horst K, Relja B, Gebhard F, Pape HC, Huber-Lang M, Hildebrand F, Kalbitz M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Shock Lippincott, Williams & Wilkins

Book Volume: 53

Pages Range: 620-629

Journal Issue: 5

DOI: 10.1097/SHK.0000000000001400

Abstract

Objective:The aim of this study was to define the influence of trauma on cardiac glucose and fatty acid transport. The effects were investigated in vivo in a porcine mono- and polytrauma model and in vitro in human cardiomyocytes, which were treated simultaneously with different inflammatory substances, mimicking posttraumatic inflammatory conditions.Methods and Results:In the porcine fracture- and polytrauma model, blood glucose concentrations were measured by blood gas analysis during an observation period of 72 h. The expression of cardiac glucose and fatty acid transporters in the left ventricle was determined by RT-qPCR and immunofluorescence. Cardiac and hepatic glycogen storage was examined. Furthermore, human cardiomyocytes were exposed to a defined trauma-cocktail and the expression levels of glucose- and fatty acid transporters were determined. Early after polytrauma, hyperglycemia was observed. After 48 and 72 h, pigs with fracture- and polytrauma developed hypoglycemia. The propofol demand significantly increased posttrauma. The hepatic glycogen concentration was reduced 72 h after trauma. Cardiac glucose and fatty acid transporters changed in both trauma models in vivo as well as in vitro in human cardiomyocytes in presence of proinflammatory mediators.Conclusions:Monotrauma as well as polytrauma changed the cardiac energy transport by altering the expression of glucose and fatty acid transporters. In vitro data suggest that human cardiomyocytes shift to a state alike myocardial hibernation preferring glucose as primary energy source to maintain cardiac function.

Authors with CRIS profile

Miriam Kalbitz

Involved external institutions

Universität Ulm DE Germany (DE) Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen DE Germany (DE) Goethe-Universität Frankfurt am Main DE Germany (DE) Universitätsspital Zürich (USZ) CH Switzerland (CH)

How to cite

APA:

Lackner, I., Weber, B., Knecht, D., Horst, K., Relja, B., Gebhard, F.,... Kalbitz, M. (2020). Cardiac glucose and fatty acid transport after experimental mono- And polytrauma. Shock, 53(5), 620-629. https://doi.org/10.1097/SHK.0000000000001400

MLA:

Lackner, Ina, et al. "Cardiac glucose and fatty acid transport after experimental mono- And polytrauma." Shock 53.5 (2020): 620-629.

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