CD11c(+) dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis

Hackstein H, Kalina A, Dorn B, Keil IS, Baal N, Michel G, Brendel C, Neubauer A, Jakob T, Bein G (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Clinical and Experimental Immunology Wiley-Blackwell

DOI: 10.1111/cei.13539

Abstract

Extracorporeal photopheresis (ECP) represents one of the most widespread and effective cell therapies for graft-versus-host disease and other T cell-mediated disorders. However, the key factors affecting the therapeutic efficacy of ECP remain unclear. We hypothesized that therapeutic effects are mediated by ECP-treated antigen-presenting dendritic cells (DC). To test this hypothesis, we used the experimental model of contact hypersensitivity (CHS). The ECP's therapeutic activity improved when the total cell dose of the ECP-treated cells was increased. We used different haptens during sensitization to demonstrate that the anti-inflammatory activity of ECP is antigen-specific. This confirmed the hypothesis that professional antigen-presenting cells are involved in the mode of action. Also, the ECP's therapeutic activity was abrogated by the depletion of CD11c(+) DC, which represents fewer than 1% of all the ECP-exposed cells. Finally, we confirm the critical importance of CD11c(+) DC for ECP activity by showing that only a few purified CD11c(+) DC are sufficient to mediate its therapeutic effect. The finding that ECP-treated, physiological antigen-presenting DC alone mediate antigen-specific modulation of a pathological immune response may result in better-targeted interventions when treating patients.

Authors with CRIS profile

Holger Hackstein Professur für Transfusionsmedizin und Cell-Engineering

Involved external institutions

Philipps-Universität Marburg DE Germany (DE) Justus-Liebig-Universität Gießen DE Germany (DE)

How to cite

APA:

Hackstein, H., Kalina, A., Dorn, B., Keil, I.S., Baal, N., Michel, G.,... Bein, G. (2020). CD11c(+) dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis. Clinical and Experimental Immunology. https://doi.org/10.1111/cei.13539

MLA:

Hackstein, Holger, et al. "CD11c(+) dendritic cells mediate antigen-specific suppression in extracorporeal photopheresis." Clinical and Experimental Immunology (2020).

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