Methods for the Administration of EDAR Pathway Modulators in Mice
Schuepbach-Mallepell S, Kowalczyk-Quintas C, Dick A, Eslami M, Vigolo M, Headon DJ, Cheeseman M, Schneider H, Schneider P (2021)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2021
Journal
Publisher: Humana Press Inc.
Edited Volumes: The TNF Superfamily
Series: Methods in Molecular Biology
Book Volume: 2248
Pages Range: 167-183
DOI: 10.1007/978-1-0716-1130-2_12
Abstract
Genetic deficiency of ectodysplasin A (EDA) causes X-linked hypohidrotic ectodermal dysplasia, a congenital condition characterized by the absence or abnormal formation of sweat glands, teeth, and several skin appendages. Stimulation of the EDA receptor (EDAR) with agonists in the form of recombinant EDA or anti-EDAR antibodies can compensate for the absence of Eda in a mouse model of Eda deficiency, provided that agonists are administered in a timely manner during fetal development. Here we provide detailed protocols for the administration of EDAR agonists or antagonists, or other proteins, by the intravenous, intraperitoneal, and intra-amniotic routes as well as protocols to collect blood, to visualize sweat gland function, and to prepare skulls in mice.
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Switzerland (CH)
United Kingdom (GB)How to cite
APA:
Schuepbach-Mallepell, S., Kowalczyk-Quintas, C., Dick, A., Eslami, M., Vigolo, M., Headon, D.J.,... Schneider, P. (2021). Methods for the Administration of EDAR Pathway Modulators in Mice. In Jagadeesh Bayry (Eds.), The TNF Superfamily. (pp. 167-183). Humana Press Inc..
MLA:
Schuepbach-Mallepell, Sonia, et al. "Methods for the Administration of EDAR Pathway Modulators in Mice." The TNF Superfamily. Ed. Jagadeesh Bayry, Humana Press Inc., 2021. 167-183.
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