Afferent renal innervation in anti-Thy1.1 nephritis in rats

Rodionova K, Veelken R, Hilgers KF, Paulus EM, Linz P, Fischer MJ, Schenker M, Reeh P, Tiegs G, Ott C, Schmieder R, Schiffer M, Amann KU, Ditting T (2020)

Publication Type: Journal article

Publication year: 2020

Journal

American Journal of Physiology-Renal Physiology American Physiological Society

Book Volume: 319

Pages Range: F822-F832

Journal Issue: 5

DOI: 10.1152/ajprenal.00063.2020

Abstract

Afferent renal nerves exhibit a dual function controlling central sympathetic outflow via afferent electrical activity and influencing intrarenal immunological processes by releasing peptides such as calcitonin gene-related peptide (CGRP). We tested the hypothesis that increased afferent and efferent renal nerve activity occur with augmented release of CGRP in anti-Thy1.1 nephritis, in which enhanced CGRP release exacerbates inflammation. Nephritis was induced in Sprague-Dawley rats by intravenous injection of OX-7 antibody (1.75 mg/kg), and animals were investigated neurophysiologically, electrophysiologically, and pathomorphologically 6 days later. Nephritic rats exhibited proteinuria (169.3 ± 10.2 mg/24 h) with increased efferent renal nerve activity (14.7 ± 0.9 bursts/s vs. control 11.5 ± 0.9 bursts/s, n = 11, P < 0.05). However, afferent renal nerve activity (in spikes/s) decreased in nephritis (8.0 ± 1.8 Hz vs. control 27.4 ± 4.1 Hz, n = 11, P < 0.05). In patch-clamp recordings, neurons with renal afferents from nephritic rats showed a lower frequency of high activity following electrical stimulation (43.4% vs. 66.4% in controls, P < 0.05). In vitro assays showed that renal tissue from nephritic rats exhibited increased CGRP release via spontaneous (14 ± 3 pg/mL vs. 6.8 ± 2.8 pg/ml in controls, n = 7, P < 0.05) and stimulated mechanisms. In nephritic animals, marked infiltration of macrophages in the interstitium (26 ± 4 cells/mm2) and glomeruli (3.7 ± 0.6 cells/glomerular cross-section) occurred. Pretreatment with the CGRP receptor antagonist CGRP8-37 reduced proteinuria, infiltration, and proliferation. In nephritic rats, it can be speculated that afferent renal nerves lose their ability to properly control efferent sympathetic nerve activity while influencing renal inflammation through increased CGRP release.

Authors with CRIS profile

Kristina Rodionova Department of Medicine 4 – Nephrology and Hypertension Roland Veelken Medizinische Fakultät Karl Friedrich Hilgers Professur für Innere Medizin (Hochdruckforschung) Peter Linz Institute of Radiology Peter Reeh Professur für Physiologie Christian Ott Department of Medicine 4 – Nephrology and Hypertension Roland Schmieder Professur für Innere Medizin (Nephrologie) Mario Schiffer Lehrstuhl für Innere Medizin IV Kerstin Ute Amann Department of Nephropathology

Involved external institutions

Medizinische Universität Wien AT Austria (AT) Universitätsklinikum Hamburg-Eppendorf (UKE) DE Germany (DE)

How to cite

APA:

Rodionova, K., Veelken, R., Hilgers, K.F., Paulus, E.M., Linz, P., Fischer, M.J.,... Ditting, T. (2020). Afferent renal innervation in anti-Thy1.1 nephritis in rats. American Journal of Physiology-Renal Physiology, 319(5), F822-F832. https://doi.org/10.1152/ajprenal.00063.2020

MLA:

Rodionova, Kristina, et al. "Afferent renal innervation in anti-Thy1.1 nephritis in rats." American Journal of Physiology-Renal Physiology 319.5 (2020): F822-F832.

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