Cd4+ T cells induced by tuberculosis subunit vaccine h1 can improve the hiv-1 env humoral response by intrastructural help
Klessing S, Temchura V, Tannig P, Peter AS, Christensen D, Lang R, Überla K (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 8
Pages Range: 1-20
Article Number: 604
Journal Issue: 4
Abstract
The induction of a potent and long-lasting, broadly neutralizing antibody response is one of the most promising approaches in HIV-1 vaccination. Recently, we demonstrated that Gagspecific T helper cells induced by DNA priming can enhance and modulate the HIV Env-specific B cell response upon virus-like particle (VLP) boost by intrastructural help (ISH). In order to minimize the induction of potentially harmful HIV specific TH cells, we explored the possibility to harness the heterologous TH cells induced by a recombinant tuberculosis subunit vaccine H1, which contains a fusion protein of Ag85B and ESAT-6 antigens in combination with the liposomal adjuvant CAF01. To provide ISH, immunodominant MHC-II restricted peptides from the H1 vaccine were genetically incorporated into the HIV 1 Gag protein and used for HIV VLP production. ISH effects on Envspecific antibody levels and B cell differentiation were analyzed in mice primed against H1 and boosted with VLPs. In contrast to non-primed mice, a significant increase of Env-specific IgG levels for up to 26 weeks after the last immunization was observed. This increase was largely caused by elevated IgG2b and IgG2c levels in mice that received H1 priming. Additionally, ISH enhanced the frequency of Env-specific long-lived plasma cells in the bone marrow. In this study, we were able to demonstrate that a heterologous prime-boost regimen consisting of the H1 tuberculosis subunit vaccine and T helper epitope modified HIV-1 VLPs resulted in enhanced HIV Env antibody and B cell responses, mediated by intrastructural help.
Authors with CRIS profile
Vladimir Temchura
Institute of Clinical and Molecular Virology
Roland Lang
Professur für Angeborene Immunität und Pathogenerkennung
Klaus Überla
Lehrstuhl für Klinische und Molekulare Virologie
Involved external institutions
Denmark (DK)How to cite
APA:
Klessing, S., Temchura, V., Tannig, P., Peter, A.S., Christensen, D., Lang, R., & Überla, K. (2020). Cd4+ T cells induced by tuberculosis subunit vaccine h1 can improve the hiv-1 env humoral response by intrastructural help. Vaccines, 8(4), 1-20. https://doi.org/10.3390/vaccines8040604
MLA:
Klessing, Stephan, et al. "Cd4+ T cells induced by tuberculosis subunit vaccine h1 can improve the hiv-1 env humoral response by intrastructural help." Vaccines 8.4 (2020): 1-20.
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