Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker

Polisetti N, Schlötzer-Schrehardt U, Reinhard T, Schlunck G (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 10

Article Number: 17588

Journal Issue: 1

DOI: 10.1038/s41598-020-74869-1

Abstract

Limbal melanocytes (LM) are located in the basal epithelial layer of the corneoscleral limbus and interact with adjacent limbal epithelial progenitor cells. The exploration of their biological role in the maintenance of the limbal stem cell niche has been limited by the difficulty of LM isolation and cultivation. Here, we report on a facile protocol for the efficient isolation and enrichment of pure populations of human LMs by fluorescence-activated cell sorting (FACS) using antibodies raised against the cell surface marker CD117 (c-Kit). The enriched LMs retain self-renewal capacity and sustained melanin production, and are suitable to study the potential of LMs in stem cell-based corneal tissue engineering.

Authors with CRIS profile

Ursula Schlötzer-Schrehardt Medizinische Fakultät

Additional Organisation(s)

Department of Ophthalmology

Involved external institutions

Albert-Ludwigs-Universität Freiburg DE Germany (DE)

How to cite

APA:

Polisetti, N., Schlötzer-Schrehardt, U., Reinhard, T., & Schlunck, G. (2020). Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-74869-1

MLA:

Polisetti, Naresh, et al. "Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker." Scientific Reports 10.1 (2020).

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