Suitability of viability assays for testing biological effects of coated superparamagnetic nanoparticles

Baehring F, Schlenk F, Wotschadlo J, Buske N, Liebert T, Bergemann C, Heinze T, Hochhaus A, Fischer D, Clement JH (2013)

Publication Type: Journal article

Publication year: 2013

Journal

IEEE Transactions on Magnetics Institute of Electrical and Electronics Engineers (IEEE)

Book Volume: 49

Pages Range: 383-388

Article Number: 6392350

Journal Issue: 1

DOI: 10.1109/TMAG.2012.2222635

Abstract

The aim of this study was to establish an in vitro model test system for the analysis of cellular effects of nanoparticles on endothelial cells using the human brain microvascular endothelial cell line HBMEC, a representative of the blood-brain barrier. At first we defined a toxic end point by using superparamagnetic iron oxide nanoparticles coated with cationic substances (polyethylenimine, chitosan, diethylamine ethyl). To study the toxic effects of nanoparticles we evaluated three different viability assays and one cytotoxicity assay for their suitability. We could show that a luminescence-based assay is most promising for an accurate testing, because it is reproducible and unaffected by assay conditions and reagents. © 1965-2012 IEEE.

Authors with CRIS profile

Dagmar Fischer

Involved external institutions

Friedrich-Schiller-Universität Jena DE Germany (DE) chemicell GmbH DE Germany (DE) Universitätsklinikum Jena DE Germany (DE)

How to cite

APA:

Baehring, F., Schlenk, F., Wotschadlo, J., Buske, N., Liebert, T., Bergemann, C.,... Clement, J.H. (2013). Suitability of viability assays for testing biological effects of coated superparamagnetic nanoparticles. IEEE Transactions on Magnetics, 49(1), 383-388. https://doi.org/10.1109/TMAG.2012.2222635

MLA:

Baehring, Franziska, et al. "Suitability of viability assays for testing biological effects of coated superparamagnetic nanoparticles." IEEE Transactions on Magnetics 49.1 (2013): 383-388.

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