Qiu Y, Rojas E, Murray RA, Irigoyen J, Gregurec D, Castro-Hartmann P, Fledderman J, Estrela-Lopis I, Donath E, Moya SE (2015)
Publication Type: Journal article
Publication year: 2015
Book Volume: 7
Pages Range: 6588-6598
Journal Issue: 15
DOI: 10.1039/c5nr00884k
Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is
APA:
Qiu, Y., Rojas, E., Murray, R.A., Irigoyen, J., Gregurec, D., Castro-Hartmann, P.,... Moya, S.E. (2015). Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs. Nanoscale, 7(15), 6588-6598. https://doi.org/10.1039/c5nr00884k
MLA:
Qiu, Yuan, et al. "Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs." Nanoscale 7.15 (2015): 6588-6598.
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