Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs
Qiu Y, Rojas E, Murray RA, Irigoyen J, Gregurec D, Castro-Hartmann P, Fledderman J, Estrela-Lopis I, Donath E, Moya SE (2015)
Publication Type: Journal article
Publication year: 2015
Journal
Book Volume: 7
Pages Range: 6588-6598
Journal Issue: 15
DOI: 10.1039/c5nr00884k
Abstract
Cerium Oxide nanoparticles (CeO2-x NPs) are modified with polymer brushes of negatively charged poly (3-sulfopropylmethacrylate) (PSPM) and positively charged poly (2-(methacryloyloxy)ethyl-trimethylammonium chloride) (PMETAC) by Atom Transfer Radical Polymerisation (ATRP). CeO2-x NPs are fluorescently labelled by covalently attaching Alexa Fluor® 488/Fluorescein isothiocyanate to the NP surface prior to polymerisation. Cell uptake, intracellular distribution and the impact on the generation of intracellular Reactive Oxygen Species (ROS) with respect to CeO2-x NPs are studied by means of Raman Confocal Microscopy (CRM), Transmission Electron Microscopy (TEM) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). PSPM and PMETAC coated CeO2-x NPs show slower and less uptake compared to uncoated Brush modified NPs display a higher degree of co-localisation with cell endosomes and lysosomes after 24 h of incubation. They also show higher co-localisation with lipid bodies when compared to unmodified CeO2-x NPs. The brush coating does not prevent CeO2-x NPs from displaying antioxidant properties. This journal is
Authors with CRIS profile
Involved external institutions
CIC biomaGUNE (Centro de Investigación Cooperativa en Biomateriales)
Spain (ES)
Universität Leipzig
Germany (DE)
Autonomous University of Barcelona (UAB) / Universitat Autònoma de Barcelona
Spain (ES)
Spain (ES)
Universität Leipzig
Germany (DE)
Autonomous University of Barcelona (UAB) / Universitat Autònoma de Barcelona
Spain (ES)
How to cite
APA:
Qiu, Y., Rojas, E., Murray, R.A., Irigoyen, J., Gregurec, D., Castro-Hartmann, P.,... Moya, S.E. (2015). Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs. Nanoscale, 7(15), 6588-6598. https://doi.org/10.1039/c5nr00884k
MLA:
Qiu, Yuan, et al. "Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2-x NPs." Nanoscale 7.15 (2015): 6588-6598.
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