Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

Prevedel R, Yoon YG, Hoffmann M, Pak N, Wetzstein G, Kato S, Schrödel T, Raskar R, Zimmer M, Boyden ES, Vaziri A (2014)

Publication Status: Published

Publication Type: Journal article

Publication year: 2014

Journal

Nature Methods Nature Publishing Group

Book Volume: 11

Pages Range: 727-730

Journal Issue: 7

DOI: 10.1038/nmeth.2964

Abstract

High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ∼700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.

Authors with CRIS profile

Tina Fraaß

Involved external institutions

Forschungsinstitut für Molekulare Pathologie / Research Institute of Molecular Pathology (IMP) AT Austria (AT) Massachusetts Institute of Technology (MIT) US United States (USA) (US)

How to cite

APA:

Prevedel, R., Yoon, Y.-G., Hoffmann, M., Pak, N., Wetzstein, G., Kato, S.,... Vaziri, A. (2014). Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy. Nature Methods, 11(7), 727-730. https://dx.doi.org/10.1038/nmeth.2964

MLA:

Prevedel, Robert, et al. "Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy." Nature Methods 11.7 (2014): 727-730.

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