Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives

Taubmann A, Willershausen I, Walter C, Al-Maawi S, Kaina B, Gölz L (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Clinical Oral Investigations Springer Verlag (Germany)

DOI: 10.1007/s00784-020-03569-x

Abstract

Objectives: The biocompatibility of methacrylate-based adhesives is a topic that is intensively discussed in dentistry. Since only limited evidence concerning the cyto- and genotoxicity of orthodontic adhesives is available, the aim of this study was to measure the genotoxic potential of seven orthodontic methacrylate-based adhesives. Materials and methods: The XTT assay was utilized to determine the cytotoxicity of Assure Plus, Assure Bonding Resin, ExciTE F, OptiBond Solo Plus, Scotchbond Universal Adhesive, Transbond MIP, and Transbond XT after an incubation period of 24 h on human gingival fibroblasts. We also performed the γH2AX assay to explore the genotoxic potential of the adhesives within cytotoxic dose ranges after an incubation period of 6 h. Results: The XTT assay showed a concentration-dependent reduction in cell viability. The decrease in cellular viability was in the same dose range most significant for Assure Plus, rendering it the adhesive material with the highest cytotoxicity. Employing the γH2AX assay, a concentration-dependent increase in H2AX phosphorylation was detected, indicating induction of DNA damage. Conclusions: For most products, a linear correlation between the material concentration and γH2AX foci was observed. The most severe effect on γH2AX focus induction was found for Transbond MIP, which was the only adhesive in the test group containing the co-initiator diphenyliodonium hexafluorophosphate (DPIHP). Clinical relevance: The data indicate that orthodontic adhesives, notably Transbond MIP, bear a genotoxic potential. Since the study was performed with in vitro cultivated cells, a direct translation of the findings to in vivo exposure conditions should be considered with great diligence.

Authors with CRIS profile

Lina Gölz Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie

Involved external institutions

Goethe-Universität Frankfurt am Main DE Germany (DE) Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) medi+ zahnärztliche Praxisklinik DE Germany (DE) Universitätsklinikum Frankfurt am Main (KGU) DE Germany (DE)

How to cite

APA:

Taubmann, A., Willershausen, I., Walter, C., Al-Maawi, S., Kaina, B., & Gölz, L. (2020). Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives. Clinical Oral Investigations. https://doi.org/10.1007/s00784-020-03569-x

MLA:

Taubmann, Andreas, et al. "Genotoxic and cytotoxic potential of methacrylate-based orthodontic adhesives." Clinical Oral Investigations (2020).

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