Helicobacter pylori HopH (OipA) and bacterial pathogenicity: Genetic and functional genomic analysis of hopH gene polymorphisms

Dossumbekova A, Prinz C, Mages J, Lang R, Kusters JG, Van Vliet AH, Reindl W, Backert S, Saur D, Schmid RM, Rad R (2006)

Publication Type: Journal article

Publication year: 2006

Journal

Journal of Infectious Diseases University of Chicago Press / Oxford University Press (OUP): Policy A1 - Oxford Open Option C

Book Volume: 194

Pages Range: 1346-1355

Journal Issue: 10

DOI: 10.1086/508426

Abstract

Background. Expression of the Helicobacter pylori outer membrane protein HopH is regulated by phase variation within a CT dinucleotide repeat motif of the hopH gene. Methods. To investigate the importance of HopH for bacterial pathogenicity, we performed a detailed functional genomic and population-based genetic characterization of this contingency locus. Results. Sequencing of hopH in H. pylori strains from 58 patients revealed that the hopH "on" genotype is linked to bacterial virulence determinants, such as the vacAs1, vacAm1, babA2, and, most strongly, cagA genotypes. hopH mutagenesis resulted in reduced bacterial adherence to gastric epithelia in vitro. Complementation of hopH in trans restored the adherence properties of hopH mutants. Although HopH has been previously linked to proinflammatory epithelial signaling, hopH mutagenesis did not alter epithelial interleukin-8 secretion in vitro. Comparative epithelial gene-expression profiling by cDNA microarrays revealed no significant differences between the wild-type-specific and hopH mutant-specific transcriptomes. By contrast, a large set of genes was differentially regulated in a cag pathogenicity island-dependent manner. Conclusion. An in-frame hopH gene may be linked to gastroduodenal diseases because of its association with other virulence factors or increased bacterial adherence and colonization. The strong linkage with cagA indicates that HopH may contribute to the fitness of cagA-positive strains in vivo. © 2006 by the Infectious Diseases Society of America. All rights reserved.

Authors with CRIS profile

Steffen Backert

Involved external institutions

Technische Universität München (TUM) DE Germany (DE) Erasmus University Medical Center (MC) NL Netherlands (NL) Erasmus University Rotterdam (EUR) / Erasmus Universiteit Rotterdam NL Netherlands (NL) Otto-von-Guericke-Universität Magdeburg DE Germany (DE)

How to cite

APA:

Dossumbekova, A., Prinz, C., Mages, J., Lang, R., Kusters, J.G., Van Vliet, A.H.,... Rad, R. (2006). Helicobacter pylori HopH (OipA) and bacterial pathogenicity: Genetic and functional genomic analysis of hopH gene polymorphisms. Journal of Infectious Diseases, 194(10), 1346-1355. https://doi.org/10.1086/508426

MLA:

Dossumbekova, Anar, et al. "Helicobacter pylori HopH (OipA) and bacterial pathogenicity: Genetic and functional genomic analysis of hopH gene polymorphisms." Journal of Infectious Diseases 194.10 (2006): 1346-1355.

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