Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells

Grainger S, Richter J, Palazon RE, Pouget C, Lonquich B, Wirth S, Grassme KS, Herzog W, Swift MR, Weinstein BM, Traver D, Willert K (2016)

Publication Type: Journal article

Publication year: 2016

Journal

Cell Reports Elsevier (Cell Press)

Book Volume: 17

Pages Range: 1595-1606

Journal Issue: 6

DOI: 10.1016/j.celrep.2016.10.027

Abstract

All mature blood cell types in the adult animal arise from hematopoietic stem and progenitor cells (HSPCs). However, the developmental cues regulating HSPC ontogeny are incompletely understood. In particular, the details surrounding a requirement for Wnt/β-catenin signaling in the development of mature HSPCs are controversial and difficult to consolidate. Using zebrafish, we demonstrate that Wnt signaling is required to direct an amplification of HSPCs in the aorta. Wnt9a is specifically required for this process and cannot be replaced by Wnt9b or Wnt3a. This proliferative event occurs independently of initial HSPC fate specification, and the Wnt9a input is required prior to aorta formation. HSPC arterial amplification occurs prior to seeding of secondary hematopoietic tissues and proceeds, in part, through the cell cycle regulator myca (c-myc). Our results support a general paradigm, in which early signaling events, including Wnt, direct later HSPC developmental processes.

Authors with CRIS profile

Wiebke Herzog

Involved external institutions

University of California, San Diego US United States (USA) (US) Eunice Kennedy Shriver National Institute of Child Health and Human Development US United States (USA) (US) Georgetown University US United States (USA) (US) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE)

How to cite

APA:

Grainger, S., Richter, J., Palazon, R.E., Pouget, C., Lonquich, B., Wirth, S.,... Willert, K. (2016). Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells. Cell Reports, 17(6), 1595-1606. https://doi.org/10.1016/j.celrep.2016.10.027

MLA:

Grainger, Stephanie, et al. "Wnt9a Is Required for the Aortic Amplification of Nascent Hematopoietic Stem Cells." Cell Reports 17.6 (2016): 1595-1606.

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