Radial glia regulate vascular patterning around the developing spinal cord

Matsuoka RL, Marass M, Avdesh A, Helker CSM, Maischein HM, Grosse AS, Kaur H, Lawson ND, Herzog W, Stainier DYR (2016)

Publication Type: Journal article

Publication year: 2016

Journal

eLife eLife Sciences Publications

Book Volume: 5

Article Number: e20253

Journal Issue: NOVEMBER2016

DOI: 10.7554/eLife.20253

Abstract

Vascular networks surrounding individual organs are important for their development, maintenance, and function; however, how these networks are assembled remains poorly understood. Here we show that CNS progenitors, referred to as radial glia, modulate vascular patterning around the spinal cord by acting as negative regulators. We found that radial glia ablation in zebrafish embryos leads to excessive sprouting of the trunk vessels around the spinal cord, and exclusively those of venous identity. Mechanistically, we determined that radial glia control this process via the Vegf decoy receptor sFlt1: sflt1 mutants exhibit the venous over-sprouting observed in radial glia-ablated larvae, and sFlt1 overexpression rescues it. Genetic mosaic analyses show that sFlt1 function in trunk endothelial cells can limit their over-sprouting. Together, our findings identify CNS-resident progenitors as critical angiogenic regulators that determine the precise patterning of the vasculature around the spinal cord, providing novel insights into vascular network formation around developing organs.

Authors with CRIS profile

Wiebke Herzog

Involved external institutions

Max Planck Institute for Heart and Lung Research / Max-Planck-Institut für Herz- und Lungenforschung DE Germany (DE) University of Massachusetts Amherst (UMass) US United States (USA) (US) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE)

How to cite

APA:

Matsuoka, R.L., Marass, M., Avdesh, A., Helker, C.S.M., Maischein, H.-M., Grosse, A.S.,... Stainier, D.Y.R. (2016). Radial glia regulate vascular patterning around the developing spinal cord. eLife, 5(NOVEMBER2016). https://doi.org/10.7554/eLife.20253

MLA:

Matsuoka, Ryota L., et al. "Radial glia regulate vascular patterning around the developing spinal cord." eLife 5.NOVEMBER2016 (2016).

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