Serum antibodies to phosphatidylcholine in MS

Cruz Sadaba M, Rothhammer V, Munoz U, Sebal C, Escudero E, Kivisakk P, Garcia Sanchez MI, Izquierdo G, Hauser SL, Baranzini SE, Oksenberg JR, Alvarez-Lafuente R, Bakshi R, Weiner HL, Quintana FJ (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Neurology, Neuroimmunology and Neuroinflammation Lippincott, Williams & Wilkins

Book Volume: 7

Journal Issue: 4

DOI: 10.1212/NXI.0000000000000765

Abstract

OBJECTIVE: To evaluate the value of serum immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies reactive with phosphatidylcholine (PC) and lactosylceramide (LC) as biomarkers in MS. METHODS: We developed an ultrasensitive ELISA technique to analyze serum IgG and IgM antibodies to LC and PC, which we used to analyze samples from 362 patients with MS, 10 patients with non-MS myelin diseases (Non-MSMYDs), 11 patients with nonmyelin neurologic diseases (Non-MYNDs), and 80 controls. MS serum samples included clinically isolated syndrome (CIS, n = 17), relapsing-remitting MS (RRMS, n = 62), secondary progressive MS (SPMS, n = 50), primary progressive MS (PPMS, n = 37), and benign MS (BENMS, n = 36). RESULTS: We detected higher levels of serum IgM antibodies to PC (IgM-PC) in MS than control samples; patients with CIS and RRMS showed higher IgM-PC levels than patients with SPMS, PPMS, and BENMS and controls. MS and control samples did not differ in serum levels of IgM antibodies reactive with LC, nor in IgG antibodies reactive with LC or PC. CONCLUSIONS: Serum IgM-PC antibodies are elevated in patients with MS, particularly during the CIS and RRMS phases of the disease. Thus, serum IgM-PC is a candidate biomarker for early inflammatory stages of MS. CLASSIFICATION OF EVIDENCE: This study provides Class III evidence that serum antibodies to PC are elevated in patients with MS. The study is rated Class III because of the case control design and the risk of spectrum bias: antibody levels in patients with MS were compared with healthy controls.

Authors with CRIS profile

Veit Rothhammer

Involved external institutions

Harvard University US United States (USA) (US) University of Seville / Universidad de Sevilla ES Spain (ES) University of California San Francisco (UCSF) US United States (USA) (US) CEU San Pablo University / Universidad CEU San Pablo ES Spain (ES) Hospital Clínico San Carlos ES Spain (ES)

How to cite

APA:

Cruz Sadaba, M., Rothhammer, V., Munoz, U., Sebal, C., Escudero, E., Kivisakk, P.,... Quintana, F.J. (2020). Serum antibodies to phosphatidylcholine in MS. Neurology, Neuroimmunology and Neuroinflammation, 7(4). https://doi.org/10.1212/NXI.0000000000000765

MLA:

Cruz Sadaba, Maria, et al. "Serum antibodies to phosphatidylcholine in MS." Neurology, Neuroimmunology and Neuroinflammation 7.4 (2020).

BibTeX: Download