Enteric Oxalate Secretion Mediated by Slc26a6 Defends against Hyperoxalemia in Murine Models of Chronic Kidney Disease

Neumeier L, Thomson RB, Reichel M, Eckardt KU, Aronson PS, Knauf F (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Journal of the American Society of Nephrology American Society of Nephrology

Book Volume: 31

Pages Range: 1987-1995

Journal Issue: 9

DOI: 10.1681/ASN.2020010105

Abstract

Background A state of oxalate homeostasis is maintained in patients with healthy kidney function. However, as GFR declines, plasma oxalate (Pox) concentrations start to rise. Several groups of researchers have described augmentation of oxalate secretion in the colon in models of CKD, but the oxalate transporters remain unidentified. The oxalate transporter Slc26a6 is a candidate for contributing to the extrarenal clearance of oxalate via the gut in CKD. Methods Feeding a diet high in soluble oxalate or weekly injections of aristolochic acid induced CKD in age- and sex-matched wild-type and Slc26a62/2 mice. qPCR, immunohistochemistry, and western blot analysis assessed intestinal Slc26a6 expression. An oxalate oxidase assay measured fecal and Pox concentrations. Results Fecal oxalate excretion was enhanced in wild-type mice with CKD. This increase was abrogated in Slc26a62/2 mice associated with a significant elevation in plasma oxalate concentration. Slc26a6 mRNA and protein expression were greatly increased in the intestine of mice with CKD. Raising Pox without inducing kidney injury did not alter intestinal Slc26a6 expression, suggesting that changes associated with CKD regulate transporter expression rather than elevations in Pox. Conclusions Slc26a6-mediated enteric oxalate secretion is critical in decreasing the body burden of oxalate in murine CKD models. Future studies are needed to address whether similar mechanisms contribute to intestinal oxalate elimination in humans to enhance extrarenal oxalate clearance.

Authors with CRIS profile

Laura Neumeier Medizinische Fakultät Kai-Uwe Eckardt Department of Medicine 4 – Nephrology and Hypertension

Involved external institutions

Yale School of Medicine US United States (USA) (US) Charité - Universitätsmedizin Berlin DE Germany (DE)

How to cite

APA:

Neumeier, L., Thomson, R.B., Reichel, M., Eckardt, K.-U., Aronson, P.S., & Knauf, F. (2020). Enteric Oxalate Secretion Mediated by Slc26a6 Defends against Hyperoxalemia in Murine Models of Chronic Kidney Disease. Journal of the American Society of Nephrology, 31(9), 1987-1995. https://doi.org/10.1681/ASN.2020010105

MLA:

Neumeier, Laura, et al. "Enteric Oxalate Secretion Mediated by Slc26a6 Defends against Hyperoxalemia in Murine Models of Chronic Kidney Disease." Journal of the American Society of Nephrology 31.9 (2020): 1987-1995.

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