Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma

Koch KN, Hartung ML, Urban S, Kyburz A, Bahlmann AS, Lind J, Backert S, Taube C, Mueller A (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Journal of Clinical Investigation American Society for Clinical Investigation

Book Volume: 125

Pages Range: 3297-3302

Journal Issue: 8

DOI: 10.1172/JCI79337

Abstract

Inflammasome activation and caspase-1-dependent (CASP1-dependent) processing and secretion of IL-1β and IL-18 are critical events at the interface of the bacterial pathogen Helicobacter pylori with its host. Whereas IL-1β promotes Th1 and Th17 responses and gastric immunopathology, IL-18 is required for Treg differentiation, H. pylori persistence, and protection against allergic asthma, which is a hallmark of H. pylori-infected mice and humans. Here, we show that inflammasome activation in DCs requires the cytoplasmic sensor NLRP3 as well as induction of TLR2 signaling by H. pylori. Screening of an H. pylori transposon mutant library revealed that pro-IL-1β expression is induced by LPS from H. pylori, while the urease B subunit (UreB) is required for NLRP3 inflammasome licensing. UreB activates the TLR2-dependent expression of NLRP3, which represents a rate-limiting step in NLRP3 inflammasome assembly. ureB-deficient H. pylori mutants were defective for CASP1 activation in murine bone marrow-derived DCs, splenic DCs, and human blood-derived DCs. Despite colonizing the murine stomach, ureB mutants failed to induce IL-1β and IL-18 secretion and to promote Treg responses. Unlike WT H. pylori, ureB mutants were incapable of conferring protection against allergen-induced asthma in murine models. Together, these results indicate that the TLR2/NLRP3/CASP1/IL-18 axis is critical to H. pylori-specific immune regulation.

Authors with CRIS profile

Judith Lind Lehrstuhl für Mikrobiologie Steffen Backert Lehrstuhl für Mikrobiologie

Involved external institutions

University of Zurich / Universität Zürich (UZH) CH Switzerland (CH) Leiden University NL Netherlands (NL)

How to cite

APA:

Koch, K.N., Hartung, M.L., Urban, S., Kyburz, A., Bahlmann, A.S., Lind, J.,... Mueller, A. (2015). Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma. Journal of Clinical Investigation, 125(8), 3297-3302. https://doi.org/10.1172/JCI79337

MLA:

Koch, Katrin N., et al. "Helicobacter urease-induced activation of the TLR2/NLRP3/IL-18 axis protects against asthma." Journal of Clinical Investigation 125.8 (2015): 3297-3302.

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