Molecular typing, serotyping and cytotoxicity testing of Campylobacter jejuni strains isolated from commercial broilers in Puerto Rico
Oyarzabal OA, Backert S, Williams LL, Lastovica AJ, Miller RS, Pierce SJ, Vieira SL, Rebollo-Carrato F (2008)
Publication Type: Journal article
Publication year: 2008
Journal
Book Volume: 105
Pages Range: 800-812
Journal Issue: 3
DOI: 10.1111/j.1365-2672.2008.03809.x
Abstract
Aims: Thirty Campylobacter jejuni strains isolated from fecal samples (n = 94; 32%) from 13 positive farms (n = 17; 76%) from commercial broiler chickens in Puerto Rico were analysed by molecular methods. Methods and Results: Isolates were identified with multiplex polymerase chain reaction assays, tested for their antimicrobial susceptibility and characterized with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), serotyping and bacterial cytotoxicity in mammalian cells. Isolates exhibited high resistance to vancomycin (minimum inhibitory concentration, MIC of >256 μg ml -1) and trimethoprim (MIC of >32 μg ml-1); few were resistant to clindamycin (MIC90 4 μg ml-1), erythromycin (MIC90 8 μg ml-1) and tetracycline (MIC90 8 μg ml-1); but none was resistant to azithromycin (MIC90 4 μg ml-1), ciprofloxacin (MIC 90 1 μg ml-1) or gentamycin (MIC90 4 μg ml-1). Most strains restricted with SmaI, but a combination of SmaI-KpnI digestion was more discriminatory. MLST analysis yielded four sequence types (ST), and ST-2624 was the predominant one. Phylogenetic analysis revealed a high degree of recombination for glnA and pgm genes. The predominant serotypes were O:3 and O:5. Most strains had lowest cytotoxicity potential with Caco-2 cells, medium cytotoxicity with INT-407 and Hep-2 cells and high cytotoxicity with CHO cells. Conclusion: A low degree of antimicrobial resistance, 13 PFGE profiles, 4 ST and a large variability in cytotoxicity assays were found for these strains. Significance and Impact of the Study: This is the first characterization of C. jejuni strains isolated from broilers in Puerto Rico. The genetic diversity of these strains suggests that several techniques are needed for strain characterization. © 2008 The Authors.
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Involved external institutions
Alabama Agricultural and Mechanical University (AAMU) / Alabama A&M University
United States (USA) (US)
Auburn University
United States (USA) (US)
University of the Western Cape (UWC)
South Africa (ZA)
Universidade Federal do Rio Grande do Sul (UFRGS)
Brazil (BR)
University of Turabo
United States (USA) (US)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
United States (USA) (US)
Auburn University
United States (USA) (US)
University of the Western Cape (UWC)
South Africa (ZA)
Universidade Federal do Rio Grande do Sul (UFRGS)
Brazil (BR)
University of Turabo
United States (USA) (US)
Otto-von-Guericke-Universität Magdeburg
Germany (DE)
How to cite
APA:
Oyarzabal, O.A., Backert, S., Williams, L.L., Lastovica, A.J., Miller, R.S., Pierce, S.J.,... Rebollo-Carrato, F. (2008). Molecular typing, serotyping and cytotoxicity testing of Campylobacter jejuni strains isolated from commercial broilers in Puerto Rico. Journal of Applied Microbiology, 105(3), 800-812. https://doi.org/10.1111/j.1365-2672.2008.03809.x
MLA:
Oyarzabal, O. A., et al. "Molecular typing, serotyping and cytotoxicity testing of Campylobacter jejuni strains isolated from commercial broilers in Puerto Rico." Journal of Applied Microbiology 105.3 (2008): 800-812.
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