The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin

Selbach M, Moese S, Backert S, Jungblut PR, Meyer TF (2004)

Publication Type: Journal article

Publication year: 2004

Journal

Proteomics Wiley-VCH Verlag

Book Volume: 4

Pages Range: 2961-2968

Journal Issue: 10

DOI: 10.1002/pmic.200400915

Abstract

Helicobacter pylori is one of the most wide-spread bacterial pathogens and infects the human stomach to cause diseases, such as gastritis, gastric ulceration, and gastric cancer. A major virulence determinant is the H. pylori CagA protein (encoded by the cytotoxin-associated gene A) which is translocated from the bacteria into the cytoplasm of host cells by a type IV secretion system. In the host cell, CagA is phosphorylated on tyrosine residues and induces rearrangements of the actin cytoskeleton. We have previously shown that tyrosine-phosphorylated CagA inhibits the catalytic activity of Src family kinases and induces tyrosine dephosphorylation of several host cell proteins. Here, we identified one of these proteins as ezrin by a combination of preparative gel electrophoresis, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). Specific pharmacological inhibition of Src family kinases also induces ezrin dephosphorylation. Therefore, ezrin dephosphorylation appears to be induced by CagA-mediated Src inactivation. Ezrin is the founding member of the ezrin-radixin-moesin (ERM) family of proteins which are signalling integrators at the cell cortex. Since ezrin is a component of microvilli and a linker protein between actin filaments and membrane proteins, this observation has important implications for H. pylori pathogenesis and might also help to explain the development of gastric cancer.

Authors with CRIS profile

Steffen Backert

Involved external institutions

Max-Planck-Institut für Infektionsbiologie / Max Planck Institute for Infection Biology DE Germany (DE) Otto-von-Guericke-Universität Magdeburg DE Germany (DE)

How to cite

APA:

Selbach, M., Moese, S., Backert, S., Jungblut, P.R., & Meyer, T.F. (2004). The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin. Proteomics, 4(10), 2961-2968. https://doi.org/10.1002/pmic.200400915

MLA:

Selbach, Matthias, et al. "The Helicobacter pylori CagA protein induces tyrosine dephosphorylation of ezrin." Proteomics 4.10 (2004): 2961-2968.

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