Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement

Schroeder A, Barschkies L, Jantsch J, Proff P, Gölz L, Deschner J, Kirschneck C (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Mediators of Inflammation Hindawi Publishing Corporation

Book Volume: 2020

DOI: 10.1155/2020/5802435

Abstract

Apart from periodontal ligament fibroblasts, immune cells like macrophages also play an important mediating role in orthodontic tooth movement (OTM). Upon orthodontic force application to malpositioned teeth, macrophages in the periodontal ligament get exposed to both mechanical strain and hypoxic conditions (via a compression of blood vessels). In this study, we assessed the relative impact of orthodontically induced mechanical strain and hypoxic conditions on macrophages for the mediation and regulation of OTM. Macrophages were stimulated with physiological orthodontic compressive forces of 2 g/cm(2) for 4 h and 24 h on gas-impermeable or gas-permeable cell culture plates under normoxic or hypoxic cell culture conditions. We quantified expression of genes involved in inflammation (Tnf,Il-6, andCox-2), extracellular remodelling (Mmp-9), and angiogenesis (Vegf) by RT-qPCR. Furthermore, we analysed HIF-1 alpha, prostaglandin-E2, and VEGF protein expression via immunoblotting or ELISA. Mechanical strain and oxygen supply both differentially affected expression of genes and proteins involved in inflammation and angiogenesis. In this context, we found that HIF-1 alpha protein levels were elevated by combined mechanical strain and hypoxic conditions, whereas gas-permeable plates providing sufficient oxygen supply prevented HIF-1 alpha stabilization at the protein level after pressure application on macrophages. Our results thus indicate that macrophages involved in the mediation of OTM are affected by and respond differently to hypoxic conditions and mechanical compressive strain, which occur concomitantly during OTM, than periodontal ligament fibroblasts (PDLF), thus indicating different roles of these cells in the regulation of OTM at the cellular-molecular level. We further observed that contrary to PDLF HIF-1 alpha stabilization in macrophages is rather induced via the decreased oxygen supply associated with OTM than via mechanotransduction by mechanical strain.

Authors with CRIS profile

Lina Gölz Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE) Johannes Gutenberg-Universität Mainz (JGU) DE Germany (DE)

How to cite

APA:

Schroeder, A., Barschkies, L., Jantsch, J., Proff, P., Gölz, L., Deschner, J., & Kirschneck, C. (2020). Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement. Mediators of Inflammation, 2020. https://doi.org/10.1155/2020/5802435

MLA:

Schroeder, Agnes, et al. "Role of Oxygen Supply in Macrophages in a Model of Simulated Orthodontic Tooth Movement." Mediators of Inflammation 2020 (2020).

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